Supplementary Table 2. Summary of putative S-nitrosylated cysteines under nitrosative stress in C. reinhardtii.

Átírás

1 Supplementary Table 2. Summary of putative S-nitrosylated cysteines under nitrosative stress in C. reinhardtii. Accession number: accession number in UniprotKB protein database. RefSeq accession number: accession number in NCBI protein database. Cysteine number: total number of cysteines in the full-length sequence. Cysteine position: experimental S-nitrosylated cysteine found by SNOSID method. S-NO: Cysteine found as S-nitrosylated in A. thaliana (Fares et al. (2011) Proteomics investigation of endogeneous S-nitrosylation in Arabidopsis. Bioch. Biophys. Res. Commun. 416, ). S-SG: Cysteine found as glutathionylated in C. reinhardtii (Zaffagnini et al. (2012) Glutathionylation in the photosynthetic model organism Chlamydomonas reinhardtii : a proteomic survey. Mol Cell Proteomics. 11, M ). This table is available as an Excel file in the ProteomeXchange database with identifier PXD000569

2 Accession number Description Cysteine number Cysteine position S-NO sites flanking sequences S-NO S-SG P protein 3 8 MAVDREECVYMAKLAEQAERYDE A8ILN4 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase IRVSLTEDPEFEMDPCKRLAGLGERAWGSGL A8ICB3 20S proteasome beta subunit B, type beta SGVLNDLGSGSNVDLCVITKDGVEYLRNYEY A8IHB3 26S proteasome regulatory subunit VMAKDELSGADIKAVCTEAGLLALRERRMRV A8IV67 26S proteasome regulatory subunit ARLCPNSTGADIRSVCTEAGMYAIRARRKTV A8J6Q7 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase CEEIARMPPLIFAGECRTLQSRLAKASTGDA A8JEV9 4-hydroxy-3-methylbut-2-enyl diphosphate reductase LTDFKEKGVQMVDTTCPWVAKVWNSVDTHTR Q8SAQ6 6-phosphogluconate dehydrogenase, decarboxylating ALKSERVAASKSFTSCVTPGPVAGVDKAQLI 338 QLINDVRAALYASKICSYAQGMNIIKAKSVE A8IAT4 acetohydroxy acid isomeroreductase IGLRPDSPSWAEAEACGFSKTDGTLGEVFEQ 466 NPYMHARGVAFMVDNCSYTARLGSRKWAPRF A8IX80 acetohydroxyacid dehydratase TLYKYIKNVSSASTGCVTDS A8JCR6 acetolactate synthase, small subunit FRARVVDVGDETLSLCVTGDPGKLTAMIKVM A8JCQ8 acetyl CoA synthetase AIRSLMSFGDQWVSQCDTSSLKLLGSVGEPI A8J933 acetylornithine aminotransferase AKRLVENSFADKAFFCNTGTEANEGAIKFAR A8HMQ1 aconitate hydratase KNGWPTELKAGLIGSCTNSSYEDMARAASVA X 466 FDKIGGTVLSNScGPCIGQWKRTDVPKGEAN P53498 actin 5 12 MADEGEVSALVCDNGSGMVKAGFAGDD A8IJ60 active subunit of chloroplast ClpP peptidase IMYHKANLNRIMADYCQQPLSKIEEDTDRDR Q6UKY4 acyl-carrier protein EADKIASVGDAINYICSNPMAK A8HS14 ADP-glucose pyrophosphorylase large subunit PIGGAYRLIDVPMSNCINSGISKIYILTQFN X

3 Q9LLL6 ADP-glucose pyrophosphorylase small subunit PLGANYRLIDIPVSNCLNSNVTKIYCLTQFN 133 IPVSNCLNSNVTKIYCLTQFNSASLNRHLSQ 237 KHRNSGAAITIAALPCAEKEASAFGLMKIDE 390 IYTMSRFLPPSKVMDCDVNMSIIGDGcVIKA A8J3Y6 AIR synthase-related protein AAVSLRDAMLDLGVACDGGKDSLSMAAAAGG 1098 RDVWEETSFVLERLQCAEEcVAQEQAGLKTA A8JFJ2 alanine-glyoxylate transaminase 6 47 ASEEVVAVDNAARKGCDGVLEVPSRLLMGPG A8JH72 alanine-glyoxylate transaminase YLPAVYKMVREAGGVCIADEVQTGFGRTGGH A8J4D3 alpha-amylase WNKFGGRLAWDASAICSNNPSFGGRGNPKQG 235 LNYNQDAHRQRTVNWCDSTGGTSAAFDFTTK A8HRR9 alpha-cop GDFVRIGADASGLLICPSQQTR A8IU73 alternative oxidase NICDDEMEHVKTMKACQDETVSQDIITRRED A8J173 aspartate semialdehyde dehydrogenase MAVTPLDRVANVKRMCVSTYQAASGAGAAAM A8I8X8 ATP citrate lyase, subunit B FKIGDTAGTLDNIVACKLHRPGSVGFVSKSG 389 FGGAIDDAARYFKAACDAGQSPSEFVEGLKK P06541 ATP synthase CF1 beta subunit 2 63 TIRAKNSAGTEMAVTCEVQQLLGDNCVRAVS 73 EMAVTCEVQQLLGDNCVRAVSMNPTEGLMRG A8IXF1 ATP-sulfurylase VRSTLPANQDVLAFQCRNPIHKAHYELFIRA P38482 beta subunit of mitochondrial ATP synthase MNVIGEPVDEQGPIECSEVWSIHREAPEFTE P04690 beta tubulin MREIVHIQGGQCGNQIGAKFWEVVSDEH X 354 SYFVEWIPNNVKSSVCDIPPKGLKMSATFIG X A8J1S7 bifunctional aspartate kinase/homoserine dehydrogenase NFLSSHYIPNRVIVDCTASDAPASKYMTWMQ A8I7T8 binding protein AVKAKIDARNQLETYCYNMKSTVEDKMKDKI

4 A8IPQ9 calcium-dependent protein kinase TATQILQHEWMRENGCAADQPIQLEVLSRMK A8I542 calcium-transporting ATPase, endoplasmic reticulum-type SVAVNKGSDPVADPNCELQSKECMLFAGTAI A8IED9 centromere/microtubule binding protein SCEAGDPKVTGNLIVCIDRATRLVKAQQAAG Q695H0 cgmp-dependent protein kinase LANMTDPEFKELLKACEQTDYNPGEVIAVAN P00877 Chain A, Crystal Structure Of Rubisco From Chlamydomonas Reinhar VERDKLNKYGRGLLGCTIKPKLGLSAKNYGR X 247 TGEVKGHYLNATAGTCEEMMKRAVXAKELGV 427 GNAPGAAANRVALEACTQARNEGRDLAREGG X X 459 VIRSACKWSPELAAACEVWKEIKFEFDTIDK P12113 chloroplast ATP synthase gamma chain GVNQRVRQEDVDSPLCAVRPVKSVLLVVLTG 152 RYRELTAMGVKVNLVCVGRKGAQYFARRKQY Q66YD3 chloroplast DnaJ-like protein VRKTTGVRKGTTPSTCPQCQGTGQVVSAVRT 220 TTGVRKGTTPSTCPQCQGTGQVVSAVRTPLG 243 SAVRTPLGTFQQVSLCPRCEGSGQISQPCTK A8IA39 chloroplast elongation factor G LEIIVDRLRREFKVECEVGAPQVNYREGISR Q1G2Y1 chloroplast polyribonucleotide phosphorylase THGSALFTRGETQSICVATLGSANDALRSDT Q6RBZ1 circadian RNA-binding protein CHLAMY 1 subunit C QVVQRFGVGSTEVLECPKTMVGRIIGKGGET Q6RBZ0 circadian RNA-binding protein CHLAMY 1 subunit C NITDVHVLNKGNAPGCAFVTYERWAHAEAAM A8I4S9 clathrin heavy chain FERLFAAGQYKEAAECAAESPQGQLRTRDVM A8J5T5 COP-II coat subunit CGGHTDQAVSYWARNCTGASGTTTEVLQNVI Q9FYV4 copper chaperone 2 13 MSTEVVLKVDMMCNGCVGAVQRVLGKLD 16 MSTEVVLKVDMMCNGCVGAVQRVLGKLDGVD Q4U0V8 coproporphyrinogen III oxidase 7 94 WERDASNPNAGYGITCVLEDGKVLEKAAANI Q9S7V1 coproporphyrinogen III oxidase HQPKAGSPEAELLDACRNPRVWV

5 Q84X73 CR061 protein VPGGIIMPDKATLSLCAIEDGEYKHDKIEFW A8J355 cystathionine gamma-synthase ILAISRLCHAKGAFVCVDSTFATPINTRALE A8J7T7 cysteine endopeptidase GYIRLKMGSTDAEGLCGIAMAPSYPVKTGPN X Q9FQ96 cytochrome c CHSMQYLHWRQLVGVCYTEEEAKALAAETEV A8HQA8 DEAD-box RNA helicase VLAPTRELAVQIQQECQRFGASSRIKNTVVY A8ITV1 decapping enzyme complex catalytic component GFPRGKVNEGETEATCAIREVLEETGYDIRS A8IU49 dehydrogenase PRKSGSILRTQVAEVCVAALTEPAAANKVVE A8J8B3 diaminopimelate epimerase LTMRIFNSDGSEPEMCGNGIRCLAKFVSDID A8HPL8 dihydrolipoamide dehydrogenase GLKVAVIEGHDIGGTCVNRGCVPSKALLAAS A8J1T4 dihydrolipoyl dehydrogenase 8 65 YVAAIKAAQLGLSVACVEGRGALGGTCLNVG 76 LSVACVEGRGALGGTCLNVGCIPSKALLNSS 81 VEGRGALGGTCLNVGCIPSKALLNSSHMYME A8IDM1 DNA topoisomerase I AGHEKDEDEADTVGcCTLKVENIEIMGDNKV A8JI07 dual function alcohol dehydrogenase / acetaldehyde dehydrogenase FASEFIYNKYKHTKTCGVIEHDPAGGIQKVA 668 RVYEVPELGKKATMVCIPTTSGTGSEVTPFS A8IQV3 EF-hand and p25-alpha domain containg protein AQLKLTAAQAASDQGCDVSATATDAGRQTCP A8JHX9 elongation factor TAALRITDGALVVVDCVEGVcVQTETVLRQA 372 YEGPLDDTYATAIRNCDPNGPLMVYISKMIP X 474 LDQYITKTATITKEGCDDAFPMKAMKFSVSP 728 RRVIYAAELTAQPRLCEPVYLVEIQAPEQAL A8ISZ1 elongation factor EF QITRALPDIVPAVSGCMNDSKQSVKDVATET A8J280 elongation factor-like protein QSARCGENLRLRLSGCEETDISPGFVLSSVK A8JH98 enolase 8 23 EKHGLEKKVEEVLNLCVKEKPEEPLSFMAKA X

6 A8HYM2 eukaryotic initiation factor 6 42 KALERPPEYVLKYYGCELGAQTNFDKASGTS A8I232 eukaryotic initiation factor KMSKGEVLMLNIGSMCTGARVLAVKGDLAKL A8ILL5 eukaryotic initiation factor IKSAIRAAEATSTEECPVKMKLVAPPLYVLT A8IRV6 eukaryotic initiation factor GRLGVRELKMLESRACNAISWSPQGRIIVLA A8IW20 eukaryotic initiation factor GSDKPLQFRQILLNTCQDEFEGAAEAREALV A8JBN7 eukaryotic initiation factor 5 78 AQSGTGKSSMISVSTCQLVDVQQRECQVLIL A8JDV9 F1F0 ATP synthase gamma subunit GKQNITVAVSSDRGLCGGLNSNIAKYTRALL A8JHB4 ferredoxin-dependent glutamate synthase KQALTALGCMEHRGACSADDDSGDGAGLMTQ 901 SIDQVEPAAAIMERFCTGGMSLGAISRETHE 1344 KVKLTLTGSGGQSFGCFcVKGLEVKLVGEAN A8J6Y8 ferredoxin-nadp reductase 8 83 RSVEKITGPKATGETCHIIIETEGKIPFWEG 143 SSRYGDDFDGQTASLCVRRAVYVDPETGKED 165 VDPETGKEDPAKKGLCSNFLCDATPGTEISM 170 GKEDPAKKGLCSNFLCDATPGTEISMTGPTG Q84X68 flagella membrane glycoprotein 1B IIAAANSTAAKAISGCVLSADGKALLVTLGA 1199 VLEVKAGTTAKGLASCSLAGTTLTVKLLGNN 1397 PFTSTVNGATLTKATCDTIVEVLNGGDATKP 2180 IKMQLPLTSNLGTVNCATTGIKVRVDDATDK 2810 SATGTITTTPATVDDCNKIVTFTPAKTLAAT 2828 IVTFTPAKTLAATSPCSVAGTTLTVNLDKAT Q6JIY9 flagellar adenylate kinase RALDQAAQFESSIMPCKTVLFFDCPEEEMEK A8HW56 flagellar associated protein GDTVLLKGKKRKDTVCIVLSDDTVDENKIRM 737 ENPDAMMEDEPDPVPCITKAHFEEAMKYARR A8I495 flagellar associated protein 8 54 NLLTKVGVPAENFPFCTIDPNNARVNVPDDR 184 LKSRGLKKEQLEELECAGRILEHLNKEIDVR

7 A8I748 flagellar associated protein TVKLPVDFPAAVASGCAKTEGLLASDVSCDT A8I7W0 flagellar associated protein CLAVASAAPLRNSADCVNVDLLKVTGVNVFP A8JG73 flagellar associated protein 1 85 GKMLLSAGDKTVALLCNVPKELQESYPHVKA A8IXD1 flagellar associated protein, adenosine kinase-like protein KANGCRPRVVVFTQGCDPTIVAVGGRVSRYP A8IKQ0 fructose-1,6-bisphosphatase MDDPQKMMEQCVMNVCQPGSRLKCAGYCLYS A8JCY4 fructose-1,6-bisphosphate aldolase RFAKWRAVVKIGEAGCPSTTAVLENAHGLAR Q42690 fructose-1,6-bisphosphate aldolase 6 58 SKGRGILAMDESNATCGKRLDSIGVENTEEN X 142 DKGLVPLSNTNGESWCMGLDGLDKRCAEYYK 256 EGILLKPAMVTPGADCKNKAGPAKVAEYTLK X A8JBY6 gamma tubulin interacting protein LDKGAGDVSEVQLNSCLRQAFTATNVVGGSS A8J3F9 GDP-D-mannose pyrophosphorylase GWDSKVGAWSRLENHCVLGEDVTVKDELYLN A8HNE8 geranylgeranyl reductase 9 93 RKLRVAVIGGGPSGACAAETLAKGGVETFLL 437 VFYRSNPAREAFVELCEDSYVQKMTFDSYLY A8JHE3 global transcription factor QEAEAAVKAGQYAMICLSDRGYGPNRAPVPS 1242 ITLGCIMMRKCHTNTCPVGVATQDPELRAKF A8IWA6 glutamate synthase, NADH-dependent QEAEAAVKAGQYAMICLSDRGYGPNRAPVPS 1237 ITLGCIMMRKCHTNTCPVGVATQDPELRAKF 1973 GKKVVVIGGGDTGTDCIATSLRHGATSIVNL Q39566 glutamate-1-semialdehyde aminotransferase ALKAQIDKGTSFGAPCELENVLAKMVIDRVP 287 LCFDEVMTGFRIAKGCAQEHFGITPDLTTMG E9N731 glutamyl-trna reductase binding protein AAAVEMSNLKSGVNSCSLMVQAATQPARAVG A8I7Q4 glutaredoxin, CGFS type 4 72 KASIAKDKGTPDSPQCGFSRMACVVLNAYGV A8IYH1 glutaredoxin, CPYC type 3 71 IAGKYTVVELENRADCDAMQDALLDITGGRS A8JFT3 glyceraldehyde 3-phosphate dehydrogenase APVKDTPAVLNVVVGCNDDKYDPAVDHIVTA

8 311 TDYVNDARSSIVDAACTQVIDGTMVKIYAWY A8JHS0 glyceraldehyde 3-phosphate dehydrogenase, minor splicing variant DEDVVSSDFVTDPASCTVDAKAGIMLSPTFV A8IYT1 glyceraldehyde 3-phosphate dehydrogenase, nonphosphorylating ISNPSTRQTAYQVQACTQDEVNKMFESAKVA A8IXZ1 glycerate kinase GQKTALVLGISAPQGCGKTTIVEQLELLFNW A8IVM9 glycine cleavage system, P protein KFLVSSKcHPQTIAVCQTRAEGLGLEAVVVD A8IWJ3 glycine cleavage system, T protein DGKVVGEITSGAFSPCLKKNIAMGYVDKDFA A8IZS5 glycine-rich RNA-binding protein 2 60 GFGFVTLEANAAKSACSECDGTEFMGRTIRV 63 FVTLEANAAKSACSECDGTEFMGRTIRVNEA B2XYE5 guanine nucleotide-binding protein beta subunit-like KYTIGEPEGHTEWVSCVRFSPMTTNPIIVSG A8JEU4 heat shock protein 70A EVEEFEHHLKELEGVCNPIITRLYQGGAGAG Q39603 heat shock protein 70B KHIDTQLTRAKFEEMCNDLLERCKVPVQQAL A8J3P5 heat shock protein 70E APKKKKVKKTDVPVQCVGAcGYSKSQLDDFF 560 KKVKKTDVPVQCVGACGYSKSQLDDFFEKEC A8J1U1 heat shock protein 90A EKKRKEELASQFEPLCRLMKDILGDKVEKVM Q66T67 heat shock protein 90C RVEKVTVSNRLLDSPCALVTSKFGWSANMER A8JE98 heterogeneous nuclear ribonucleoprotein 1 29 MAGGVQGQIGPDGFPCVRLRGLPFDVMEGDI A8I3J7 homoserine dehydrogenase AAEADAAGGVVRYVGCVDAENGNASVTLRTY A8I826 homoserine kinase MMAVKEAAKAAGAYGCTISGAGPTAVAIVDD A8J841 hydroxymethylpyrimidine phosphate synthase KAEDLTFRQAPTIADCFPDSEKMYKEVKYDD 218 RAIIPANKcHLELEPCVIGRNFMTKVNSNFG 581 PAKTAHFCSMCGPKFCSMNITQELRQMVQAE A8J3Y3 hypothetical protein CHLREDRAFT_ GGAAAAAAARDDNPPCNTLFVGNLSDSVDEN

9 A8JGP5 Ribulose bisphosphate carboxylase small chain 4 64 AYVSNESAIRFGSVSCLYYDNRYWTMWKLPM Q84X75 CR051 protein 3 54 LTKTTAREYSGRTITCNAVAPGFIASDMTAA A8II85 hypothetical protein CHLREDRAFT_ ARAKQQRITPDVQTGCIScASGDGK A8IS20 hypothetical protein CHLREDRAFT_ LEGWRAAGLDVQGIVCDVSDRAQRGELAAKV A8JBL6 hypothetical protein CHLREDRAFT_ DIPLKQKIFADLEKACRPDAILSTNTSTIDI Q84X77 CR027 protein QRSPPRPAGGSSAGGCGcGSSGGGGGGCKSG A8I720 hypothetical protein CHLREDRAFT_ NMRRLDSLRHRVRVLCPSVEANEITTEAEAR A8IH45 hypothetical protein CHLREDRAFT_ YVLKGAQRTSGSTQSCCPAGNIAATLPSGRR 268 VLKGAQRTSGSTQSCCPAGNIAATLPSGRRL A8ISA5 hypothetical protein CHLREDRAFT_ SPMFKKGWKKTIIDMCYSPPVDGAKAVIHAA A8JIU3 hypothetical protein CHLREDRAFT_ AKRIESITWHLKDRGCDNFDVVEKATTSPAS A8IRL5 hypothetical protein CHLREDRAFT_ SAAATEGASNAVPADCVSPEGRMLVFTLADS A8HVL0 hypothetical protein CHLREDRAFT_ WEVTARDFADKADEVCVQSVDDIGKVYKKVQ A8IJD8 hypothetical protein CHLREDRAFT_ STVSLARSTVPGEDVCIGAGTSIGEGSRVVQ A8INI0 hypothetical protein CHLREDRAFT_ ADLDLGYNEIKDDGACALAQALKANPEGAPR A8IT30 hypothetical protein CHLREDRAFT_ AELAKEHEARAAGGECGGGTGEAEcPRVRLV A8J654 hypothetical protein CHLREDRAFT_ AIDAVLARSTGPTGDCYSFERGN A8J7Z6 hypothetical protein CHLREDRAFT_ IPFDATVRISLDTNLCMIKENPEDGPSCAAT A8J725 hypothetical protein CHLREDRAFT_ QELIPLVKFMGNTGGCEPQAWSKDPPLVYIN A8J3W1 hypothetical protein CHLREDRAFT_ APMLTDEEQAAEDAACAKVIDTVFNAAKPAS

10 A8J538 hypothetical protein CHLREDRAFT_ RTALADTPANRFVNDCTNLVQEQRYEEYVTR A8J5B8 hypothetical protein CHLREDRAFT_ STIKAAELEAVGSAVCAKVHTVTHPGDLWLK X 248 TALEAFHHMALDHKSCLGIVDNTGKLIGSVS X Q6PLP7 hypothetical protein CHLREDRAFT_ WQPCKADGSGFETEGCGELTCDGKTADHGNL 639 ADGSGFETEGCGELTCDGKTADHGNLRRKGT A8HWI6 hypothetical protein CHLREDRAFT_ IGSDLRFGVQLQTMTCDTPKVYKIHDKLYLG A8JAG1 hypothetical protein CHLREDRAFT_ KVALLEKETFPRDKYCGDAVCTPAIRILEDM 107 EKETFPRDKYCGDAVCTPAIRILEDMGVLQE 216 VTSATGTVVRGRVLVCADGATSRLATKLGYC 231 CADGATSRLATKLGYCTEAPKGICSRAFIEG A8JAP7 Inosine-5'-monophosphate dehydrogenase SKLYELKNTRGFTSVCVTDTGALGGKLLGVV 326 AGADGLRVGMGSGSICTTQEVCAVGRGQATA 332 RVGMGSGSICTTQEVCAVGRGQATAVYHVAR A8JAQ6 hypothetical protein CHLREDRAFT_ KTLEEVVHEAGAASGCPKAKGNLLYTVASKY A8JFZ2 hypothetical protein CHLREDRAFT_ ADLYGGIGDVSATGDCAGDAKALCKDVNSGD A8JJD2 hypothetical protein CHLREDRAFT_ GLLNRSRDIMRQGATCFPDDQVEAKKALARW A8IZ88 hypothetical protein CHLREDRAFT_ KALIEAMRRGASVAECVQVIESAGGSK A8JG58 hypothetical protein CHLREDRAFT_ VKAKLEAGQTATVSNCDGQTFEIKAGMVEIK A8ISA8 hypothetical protein CHLREDRAFT_ AAGSGGAAATSSGGDCRVLIYGGYSGEAVEG A8J742 hypothetical protein CHLREDRAFT_ TVIRQKQAIRDELQSCDATRESIRAEMRSIR A8INX1 inactive subunit of chloroplast ClpP complex MKFTGMNQDQIEQATCRDHFMTPEQAKLEGL Q93Y52 inorganic pyrophosphatase WKVIAISADDPKAALCNDVEDVEKHFPGEIQ A8IXE2 intraflagellar transport particle protein SKKTQAGGTDVFAVACTDGSVKILSRTGRVE

11 Q39577 isocitrate lyase NAYELMKALIEAGASCVHFEDQLASAKKCGH 247 DKPFMTGERTAEGFYCVRGGIDAAIARGLAY 301 AAIHAQYPGKLLAYNCSPSFNWKKLSADEIS A8JG03 isopropylmalate dehydratase, large subunit GSAIEGMNMDERMTICNMVVEAGGKNGVIAP 402 TQKVWADVYTMPVPGCDGKTAAEIFEESGCI 444 GGPRDTFARMNEPEVCVSTTNRNFPGRMGHK X A8HYG8 isopropylmalate dehydratase, small subunit MKTEYPIIIGGQNFGCGSSREHAPVALGAAG A8HXS9 isopropylmalate synthase ANSLAGAQAGARQIECTINGIGERAGNASLE A8JG56 L-ascorbate peroxidase EAAIKEVVTTEDGPLCVRLVLNDAADWDPVT A8IT07 LCIB-like gene RIARRLAYEKINPLDCSLVDVTKAAERVISA A2SY49 light-harvesting chlorophyll-a/b binding protein Lhcb GAEFYRNTETDPEKRCYPGGVFDPLKLASED A2SY45 light-harvesting chlorophyll-a/b binding protein LhcbM2b 1 76 LELIHARWAMLGALGCITPELLAKNGIPFGE Q75NZ1 low-co2 inducible protein LCIC LMAARSLADVPPGDICAGQRGSVLQEIPYGY A8J7C8 lysyl-trna synthetase, LysRS NSDLDAEGYAQVHNECKRGDIVGVEGFPGKS 502 NMTERFELFINKREVCNAYTELNDPLKQREL A8IMZ5 magnesium chelatase subunit I QGEVNYDFRVKISQICSDLNVDGIRGDIVTN P93106 malate dehydrogenase PVKVTGYTGPEELGACLKGADLIVIPAGVPR A8IL08 membrane AAA-metalloprotease QEVPETGVVFDDVAGCDGAKLELQEVVDFLK A8J3U9 mitochondrial ATP synthase subunit 5, OSCP subunit LAYEELMLAHKKEVHCTVVTAQPLDDAERAV A8HTX7 mitochondrial F1F0 ATP synthase associated 31.2 kda protein AAFLEDVKKVQWFDACVAENPAVGPKVTA Q96550 mitochondrial F1F0 ATP synthase, alpha subunit ARVYGLKSVQAGELVCFDSGVKGMALNLQAD 527 TKGYLDKVPVNQITACEDVILKHVDPRLFKI

12 A8J236 molybdopterin biosynthesis protein GPAPRDVTPEATEAVCSRMMPGYGEQMRAIS Q6VPQ2 MPBQ/MSBQ methyltransferase DPNLKVVDVGGGTGFCTLGVVKTVKPENVTL A8J980 mrna export protein 6 90 ASAWKNDGSGIFLAGCDKAVRLWDLASNQAV A8JGS3 MYG1/GAMM1-like protein YCVYQDDRDKSYRVQCVSVGPGSFENRRSLP A8HWC2 NAD malic enzyme VMGLPPAALGQQRIVCVGAGSAGMGVARMIA A8JCP5 NAD(P) transhydrogenase VEALAKRRATAIGMDCIPRTISRAQMFDSLS Q6V9A9 NADH:ubiquinone oxidoreductase 11 kda subunit 2 53 EMKKANPHFPILIRECAGTEASLTARYDFGA A8IHL3 NADH:ubiquinone oxidoreductase ND9 subunit HLLSPRWNNRIRVKVCVDEVTAVPTLCKVFN 195 RVKVCVDEVTAVPTLCKVFNTANWFERETWD P93104 NADP-malate dehydrogenase 3 74 ESVSRNCKVLVVGNPCNTNALIAMENAPNIP A8J544 nucleolar protein, component of C/D snornps SRYLANKCSIASRIDCFMDGNTNVFGEKMRE A8I9L3 nucleoredoxin MSDKPADVICGDVCRRVTPEEAAAR Q39580 outer dynein arm light chain KNADMSEEMQADAVDCATQALEKYNIEKDIA B1B601 Parkin-co-regulated gene product 3 35 AGKAPPAANLSGTGSCFDTTSLSPARAGAHK A8HUU9 peptidyl-prolyl cis-trans isomerase, cyclophilin-type GSRSGETAFDVMVADCGELPAGAARAAAPRQ A8IVQ7 peptidyl-prolyl cis-trans isomerase, cyclophilin-type 8 95 FMIQGGDFVKGDGTGCVSIYGSRFPDENFIA A8JD64 peptidyl-prolyl cis-trans isomerase, cyclophilin-type GSSSGKTRATIAIADCGQLA A8HN08 PfkB-type carbohydrate kinase 9 30 EKLRTEKLETQGGGNCANALTAAARLGLYPT A8J0N7 phosphoenolpyruvate carboxykinase, splice variant 9 52 RFTNNTKQDAAVPAPCDMQFVLDSKFTRESG

13 A8J8Z2 phosphoglucomutase WKFFGNLMDAGKcSVCGEESFGTGGDHIREK 439 KQYGRNFFSRYDYEECASADADKMVAHLRDV Q94KV1 phosphoglyceromutase VEELEAVLAELRGKGCDIAIASGGGRMQVTM P19824 phosphoribulokinase 5 47 DKDKTVVIGLAADSGCGKSTFMRRMTSIFGG X Q39615 photosystem I reaction center subunit II, 20 kda MRQGPNLLKFGKKEQCLALTTQLRNKFKLTP B2X290 photosystem I subunit VII [Chlamydomonas moewusii] 9 11 MAHVVKIYDTCIGCTQCVRACPLDVL 14 MAHVVKIYDTCIGCTQCVRACPLDVL 17 AHVVKIYDTCIGCTQCVRACPLDVLEMPV 54 MASAPRTEDCVGCKRCETACPTDFLSVRVYL 58 PRTEDCVGCKRCETACPTDFLSVRVYLGSES Q6J213 phytoene desaturase SRSPLLSVYADMSTTCKEYYDTEKSMLELVF Q93Z22 plasma membrane-type proton ATPase ARMSAVEEMAGMDILCSDKTGTLTLNKLSID 516 LFDPPRHDTKDTIEYCHGQGIEVKMVTGDHL A8I8Z4 plastid ribosomal protein L KPPGAKGVYWKSMYVCTTMGPSLRIGVSALQ A8JAL6 plastid ribosomal protein L EKRKGRGYAAGQGGTCGFGNRGQKARSGPSV A8J3Z3 plastid ribosomal protein L KEGIHPQWFEEAKVICNGVEVMTVGGTKATY A8IYS1 plastid ribosomal protein L IADAIYQQTGRNVSDCEITVPEIKSVGTYEC Q70DX8 plastid ribosomal protein S DDGAYVEIGAKAIAFCPVTEcSFARLKTPLE A8J8M5 plastid ribosomal protein S ctaiigngnglvgvgcqagrevatavkralv A8JCR1 PP2A-twin subunit DKTVKLWKVYEKKVSCLSNFNLEGRNNGGTA A8HN94 predicted protein 4 80 SLTLTHKGAEVVAQLCTKSLGGLTENDFIVA A8HNF0 predicted protein AGAARVIAVDGSAGACDSARRIVRANGLDDR

14 A8HP72 predicted protein 6 76 KTQVKRALETYVKTDCPSTTAPSSTVWRCMS A8HPV5 predicted protein 1 55 APLDAARMTVDAAGTCAEAARGITQYVPKPK A8HRV2 predicted protein TPMTEFRLESVPDMNCDALDAKTPKGEVLIR A8IBN3 predicted protein YIPETGRGIQGATSHCLGQNFSKMFNITYED A8IFH0 predicted protein TRLAAIEDMAGMNMLCSDKTGTLTLNKMAIQ A8IK94 predicted protein YTYKSAREALEDDPECSNPETQALARFSGAE A8ITK6 predicted protein DVFTVAGARGQNLVECPNCKADMIFDEYKRV A8ITU2 predicted protein 7 98 DLPKGYQISQYDEPICSGGRLEVEVDGVMKR A8IV25 predicted protein QALPQFQKGLGTPGLCAAEGEWVEPKKDAGK A8IVG4 predicted protein RDMVTAGQVEEARALCVAQVDEYVGKIASDF A8IXZ2 predicted protein LVRFATDNESIGAVICKRADQLQASCVVMAK A8IYA3 predicted protein 2 90 TSEMWHPNVYDDGKVCISILHSPGDDPSGYE A8IYA5 predicted protein KAADKAREAAELRAGCEAAVAADQPADVAKL A8IYX9 predicted protein 1 30 LTGVTRVEVQDTSGGCGAMYRIAIQAADFKG X A8IYZ6 predicted protein 3 29 KPANFLVDRAWKVKVCDFGLASNSKAQAGAG A8IZZ6 predicted protein 4 42 NGGRILYPGSDLPTACPVTEDKEWRASHDHA A8J0B3 predicted protein 8 63 ASVSFEQPDEASLVSCRFIVPHHVTKPGQQL 285 PLYEGSGDIQAKVVICNADGSPAVWEPGTTN A8J1C4 predicted protein 3 83 DELVLACRDKLSSGGCSPDGLSKVVSALPLL A8J2L0 predicted protein AGEGADGEAPAGDFACGDCDRTFASQQGLRR

15 117 GADGEAPAGDFACGDCDRTFASQQGLRRHLQ A8JD33 predicted protein EFLIKGAPGSTAELLCNASAGDPVSVSPVMG A8JDL8 predicted protein 6 52 SMHGLPTDRPKIERLCADIQSQHGVRVAYSE A8JDU0 predicted protein GSSSTYAITHYFNKACPSQWSAVAGSPATK A8JDV3 predicted protein LDLLEEVPYAELKALCAEAGLSAAGKKAELQ A8JF87 predicted protein ATVTKTGAGVDVALICDGSGAGRSGKDKKDV A8JFA1 predicted protein 4 93 VYGRQMGGVPEESEGCAVcKVDTGLGGQPCY A8JGW7 predicted protein PGVDVHDEAAVKDKLCADIAAAGDGDDASAA A8JH69 predicted protein SEVLFLETNSERYQLCNVSISDDPKGKDLFT A8JHA6 predicted protein 4 97 NLEEYEAAKEAFEAGCALAPDNTFKTWIRKC A8JHB7 predicted protein AQYLFQRRGPLATTGCDHGAFVRTSSSLSQP A8JHF3 predicted protein AVRAVRHRALEQAKLCNTGGPAGELKRMEQQ A8JHJ9 predicted protein TNMARMNGANGVSFGCVNTAAPVDPKEEPKKE A8JJI9 predicted protein 1 25 DLENVSRSAALINQSCHVRVLDIRKFLDGIY Q84X70 CR084 protein GVEITQAALDLGAEECSKRTTDAMRDAHKKS A8HXC5 prephenate dehydratase AQIVARQGLQGVGAICSRRAAELYGLDVLEE A8HPV3 presequence protease LKELQETPDSPEALACIPALKLSDIPKTITK A8IS98 PRLI-interacting factor L QLEAEPQPNQPAPGACPGQAARSVYLSIAES A8HXI4 protein phosphatase 2A regulatory subunit 9 24 LPSMVAYSLFKRLTGCEAGEEEGAAVPRDTF Q8LKK4 protofilament ribbon protein of flagellar microtubules 4 17 SGGAPPIPKLPGYTVCLPQSLSDKGFKKGQT

16 A8HWA8 pyridine nucleotide binding protein PAEFEAAIGSANKIVCAVGAAEDQALNFSAP A8IW49 pyruvate phosphate dikinase DAAEARKFGAEGIGLCRTEHMFFASEERIAT VERGRAARPDLKIGICGEQGGEPRTVAFLHE Q27YU3 radial spoke protein GSGGATLTESQVTGLCQQAGIADAVVAKVME Q39586 RecName: Full=5-methyltetrahydropteroyltriglutamate--homocystein PILIGPVTFVSLSRGCELPLDQAVARLLPTY Q9SW75 RecName: Full=60S ribosomal protein L10a 4 72 VPRPRMRVRAGDVKHCEQAGAIGVDAKGVED P12853 RecName: Full=Oxygen-evolving enhancer protein 1, chloroplastic; S 3 74 GLTYLQVKGSGIANTCPVLESGTTNLKELKA 98 NLKELKAGSYKLENFCIEPTSFTVKEESQFK Q6R2V6 RecName: Full=Phosphoenolpyruvate carboxylase 2; Short=PEP car GVRRDRATREPNPNSCDAVFARLITEGVDPE P41758 RecName: Full=Phosphoglycerate kinase, chloroplastic; Flags: Precu TVSIANTLAGLTPKGCITIIGGGDSVAAVEQ X P23489 RecName: Full=Ribulose bisphosphate carboxylase/oxygenase activa TRYREASDIIKKGRMCSLFINDLDAGAGRMG 289 EKYYWNPTREDRIGVCMGIFQEDNVQRREVE P46284 RecName: Full=Sedoheptulose-1,7-bisphosphatase, chloroplastic; Al CDGKAVSALDIPILVCDQRTQICYGSIGEVR 362 ALDIPILVCDQRTQICYGSIGEVRRFEEYMY P09205 RecName: Full=Tubulin alpha-2 chain TVVPGVDLAKVQRAVCMISNSTAIGEIFSRL A8J597 ribosomal protein L12, component of cytosolic 80S ribosome and MPPKFDPSDVVEVCVRVTGGEVGAASSLA 142 ANGCKEILGTAVSVGCKVDGKDPRDVISAID A8IKZ2 ribosomal protein L18, component of cytosolic 80S ribosome and FTETARARITKAGGECLTFDQLALQRPTGKD A8HN50 ribosomal protein L18a, component of cytosolic 80S ribosome and RKLRRVKKANGQIIACNEIFERKPTLAKNYG A8JI94 ribosomal protein L22, component of cytosolic 80S ribosome and AKGAAKKKALVFTIDCSKPVEDKIMDISSFE A8J9W0 ribosomal protein L23, component of cytosolic 80S ribosome and KFRMALGLPVAAVMNCADNTGAKNLYVISVV 57 VVGWGSRLNKLPAAACGDMVMASVKKGKPDL

17 A8J239 ribosomal protein L23a, component of cytosolic 80S ribosome and VNTLIRPDGQKKAYVCLTADYDALDVANKIG A8ICT1 ribosomal protein L30, component of cytosolic 80S ribosome and GVHHYGGNNVDLGTACGKYYRVSVLAITDPG Q8GUQ9 ribosomal protein L38, component of cytosolic 80S ribosome and TTKFKVRCSKYLYTLCVADSDKADKLKQSLP A8IVK1 ribosomal protein L8, component of cytosolic 80S ribosome and 60S GNVKPLGDMPEGTIVCNVEEKAGDRGALARC A8JHC3 ribosomal protein S11, component of cytosolic 80S ribosome and PCFRVREGDSVVIGQCRPLSKTVRFNVLRVT A8IGY1 ribosomal protein S13, component of cytosolic 80S ribosome and PSWCKTTGAEVQEMICKFARKGMTPSQIGIV A8HVP2 ribosomal protein S18, component of cytosolic 80S ribosome and VCKKAEVDLRKRAGECSADELERMMGIVANP A8HME4 ribosomal protein S2, component of cytosolic 80S ribosome and 40S ARTPKKVLQMAGIEDCYTCSRGSTKTLGNFV 211 PKKVLQMAGIEDCYTCSRGSTKTLGNFVRAT A8IS22 ribosomal protein S26, component of cytosolic 80S ribosome and RNIVDASALRDMQEACVVDNYALPKIYRKVY A8HVK4 ribosomal protein S27a, component of cytosolic 80S ribosome and 4 5 YKVDDSGKVQRLRKQCPNcGPGTFMATHFDR A8HS48 ribosomal protein S3a, component of cytosolic 80S ribosome and AKFIPESIGKDIEKSCQGIYPLQNTFIRKVK A8J2I5 ribosomal protein S5, component of cytosolic 80S ribosome and 40S GAREAAFRNIKTIAECLADELVNAAKGSSNR X X A8J1G8 ribosomal protein S6, component of cytosolic 80S ribosome and 40S YGRRKGERRRKSVRGCIVSPDLAVLNLVIVK X A8IB25 ribosomal protein Sa, component of cytosolic 80S ribosome and 40S 5 18 LSQKEQDIQMMLAAQCHLGTKNCHYQMERYM A8I9I9 rieske ferredoxin 5 KTALFTAEVKDKCVICPAHGTAFDVKTGEVK A8IBR0 RNA binding rrna processing protein VVVRSAGDKRNVLVGCGPGDVAGAVTKEADA A8IXE0 S-Adenosyl homocysteine hydrolase LEIDLAEAEMPGLMACRSEFGPAQPFKGAKI X A8HYU5 S-Adenosylmethionine synthetase 9 23 LYTSESVNEGHPDKICDQVSDAVLDACLEQD 34 PDKICDQVSDAVLDACLEQDPDSKVACETCC 76 TTKAKVDYEAIVRKVCKEIGFTSEDVGLDAD

18 93 EIGFTSEDVGLDADKCKVLVHIEEQSPDIGQ A8JFZ0 serine glyoxylate aminotransferase VHNETTTGVTSDIAGCRKAMDAAGHPALLLV A8JFK4 serine hydroxymethyltransferase 2 7 DLRPEGVTGSKMEKACDLCHITLNKNAVVGD A8J4R9 serine hydroxymethyltransferase SENFTSKAVMQALGSCMTNKYSEGRPNARYY 341 NTEEFRTYQKQVVANCSALCGRLQQHGYKIV A8JHT4 serine/threonine protein kinase LAKGSAETGMVEAYMCAKVKRNPLVASSCAE A8I848 seryl trna synthetase 2 49 MPGLQRYGEISSASNCTDYQSRRLNIRYSGG A8IRT6 seryl-trna synthetase EAWFPASATFRELVSCSNcLDYQSRRLDVRL A8HNU4 signal peptide peptidase-like protein KATSRQVADATADAACNAYDRIADQEALQRE A8I9S6 spliceosome component, nuclear pre-mrna splicing factor 9 3 MFCSISGTVPEQGVVSTK A8J4I2 SR protein factor GGPGGPGGREMRcYECGEIGHIARDCRNMRG A8IJ34 sterol-c24-methyltransferase FKENTFDGAYAIEATCHAPKLEQVYGEIYRV B2XYH3 striated fiber assemblin 1 24 DIEHLATSLVGKVNECVAAIDEERNGRVQEQ A8JIJ1 subunit of exon junction complex DECDKMLEKLDMRADCQEIFKLTPHEKQVMM A8HQF0 subunit of retromer complex GSEHRDALTAAATAGCMRLVARREQCRALCA A8JH66 subunit of the signal recognition particle KVKPRDSFEVAFNRSCGLVEAGQLASAEGEL A8HP06 succinate dehydrogenase subunit A LRAAVGLSELGFNTACITKLFPTRSHTVAAQ 260 GGYGRAYFSATSAHTCTGDGNAMAARAGIPL A8IQ09 succinate-coa ligase beta chain, minor isoform AKQIKALYTLFDKSDCTMVEVNPLAEGLDGS A8II42 T-complex protein 1, gamma subunit EIKESDIGTGAGLFECVKIGDEFFTFIVDCK 454 VGVAMEVIPRTLAQNCGANVIRTLTKLRAKH

19 A8J7J2 T-complex protein, epsilon subunit KKRQLEEKNPYLGIDCNDTGTNDMREQNVFE 520 TVMGKKQQLFLATQVCKMILKIDDVIKPSDY A8IYP6 thioredoxin h FLKVDVDELADVAGECGVKAMPTFIGYFNGE Q84XS0 thioredoxin o 4 67 SAKLADVAGSGSLMICDFTAKWCGPCRMIAP A8HZZ1 threonine synthase MCKELGLVDKMPRLVCAQAQNANPLYQAFSK 472 QSKVAYHSKQVPGMTCQFANPPVQVREDLGA A8J1M9 thylakoid lumenal 17.4 kda protein VWEDALIGSQDVGKLCENPTLTGESRAQVGC A8IAN1 transketolase TTIALSRQNMPNIPNCSVEGVAKGAYTIHDT C0LL59 translation elongation factor-like protein DFIKNMISGAAQADVCLLMVPADGNFTTAIQ A8ISB6 transmembrane protein of CMA family YKFSGSGTNTTLTGSCLLSKDFTNQDYCNYA Q5S7Y5 triose phosphate isomerase DKLRIIYGGSVSDTNCKDLSKQEDIDGFLVG X 273 LVGGASLKGAAFVTICNAAGPKAKP O64991 tryptophan synthase beta AETGAGQHGVATATICARLGLKCIVYMGAKD A2PZB5 UDP-glucose 4-epimerase like protein HVSAVVKTLATPDLGCTPINLGTGKGTSVLE A2PZC3 UDP-glucose dehydrogenase IGPKFLNASVGFGGSCFQKDILNLCYVCESV A2PZC2 UDP-Glucose:protein transglucosylase GRYDDMWAGWCTKVICDHLGVGCKTGLPYVW A8IW47 vacuolar ATP synthase subunit E CCGGVVLISGDGRINCSNTLDDRLKIAYQAN X X A8I164 vacuolar ATP synthase, subunit A IAIDSGDVFIPRGVACPSLDIVKQWEFQPKG 304 MTLPDGREESIMKRTCLVANTSNMPVAAREA A8J1K0 vacuolar proton translocating ATPase subunit A NDFGEPITNTIDPRDCQMVYEGVLKMPPDSA A8I647 zeta-carotene desaturase DYIDSMEGATLSGRQCAYSILNATPGIQKSL