Horizontal gene transfer drives adaptive colonization of apple trees by the fungal pathogen Valsa mali. Zhiyuan Yin, Baitao Zhu, Hao Feng, Lili Huang*

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1 Supplementary Information Horizontal gene transfer drives adaptive colonization of apple trees by the fungal pathogen Valsa mali Zhiyuan Yin, Baitao Zhu, Hao Feng, Lili Huang* State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712, Shaanxi, China *Corresponding author Lili Huang,

2 Supplementary Figures Supplementary Figure S1. Dead tissues of apple bark caused by Valsa mali in the fields.

3 Supplementary Figure S2. Bleomycin resistance assays of three fungi under different levels of bleomycin stress. were grown on the potato dextrose agar (PDA) media at 25 C in the dark for two days.

4 Supplementary Figure S3. Generation of gene deletion mutants. (a) Gene replacement strategy of hygromycin-resistant cassette. (b) Gene replacement strategy of G418-resistant cassette.

5 Supplementary Tables Supplementary Table S1. The genes involved in lysine biosynthesis in V. mali Gene Description Ortholog/homolog in V.mali a Genes involved in α-aminoadipate (AAA) pathway b LYS20/LYS21 Homocitrate synthase VM1G_07016 LYS4 Homoaconitase VM1G_10252 LYS12 Homoisocitrate VM1G_06170 dehydrogenase LYS2 α-aminoadipate reductase VM1G_02319, VM1G_105, VM1G_06405, VM1G_03154, VM1G_05645 LYS9 Saccharopine reductase VM1G_06828 LYS1 Saccahropine dehydrogenase VM1G_01424 Genes involved in succinylase (DAP) pathway c Ask Aspartate kinase VM1G_06620 Asd Aspartate semialdehyde VM1G_082 dehydrogenase DapA Dihydrodipicolinate VM1G_02804 synthase DapB Dihydrodipicolinate NA reductase DapD THDPA succinylase NA DapC succinyl-dap VM1G_011 aminotransferase DapE succinyl-dap VM1G_00056 desuccinylase DapF DAP epimerase NA LysA DAP decarboxylase VM1G_069 a Orthologs to genes involved in AAA pathway of Saccharomyces cerevisiae were identified by orthagogue (Ekseth O K, Kuiper M, Mironov V. orthagogue: an agile tool for the rapid prediction of orthology relations. Bioinformatics, 2013: btt582.); homologs to genes involved in DAP pathway were identified by blastp searches using known bacterial genes. b Refer to "Xu H, Andi B, Qian J, et al. The α-aminoadipate pathway for lysine biosynthesis in fungi. Cell biochemistry and biophysics, 2006, 46(1): " c Refer to "Liu Y, White R H, Whitman W B. Methanococci use the diaminopimelate aminotransferase (DapL) pathway for lysine biosynthesis. Journal of bacteriology, 2010, 192(13): "

6 Supplementary Table S2. Primers used for gene deletion and qrt-pcr. Names Sequences (5'-3') HGT32-1F CCATTGCGGAGAAGGTTAG HGT32-2R CAGATACGGCAGAGAAATCGCAACCTCCAAATGCTGCGTGGGTAA HGT32-3F GTTTAGATTCCAAGTGTCTACTGCTGGCTGGGTAGCGTTGTAGTTGT ATG HGT32-4R TGAGAAGGGAGCCAGCAT HGT32-5F ACGAATAACTCGCCTGTGG HGT32-6R AGCCGGGTCCTGAAATG HGT32-7F GCCAGAGCCAAGTCAACC HGT32-8R GCCCGTCCAGTATGTAATCA HGT32-CF GCAACAACCGAAGACGAAA HGT32-CR CTGACAAGGCAGGAGGAGA G852-F TCGGCTATGACTGGGCACAACA G850-R GAGCGGCGATACCGTAAAGCAC G856-F GAATGGTCAAATCAAACTGCTAGATAT G855-F TGTTGGGTTTGAGCTAGGTGGG HGT32-qPCR-F TGGACGATGTAGACGAGGTAT HGT32-qPCR-R TCCTGAAATGTCACAGTCCTAAC HGT14-1F GGCTCCAAATCCCCTCG HGT14-2R TTGACCTCCACTAGCTCCAGCCAAGCCAATACCACTGCTAATACAA TGCTG HGT14-3F GAATAGAGTAGATGCCGACCGCGGGTTGGGAAAGACTACGCCGAA TG HGT14-4R GCGTCCATAGAGCGGAAGAT HGT14-5F CGTCACTGCCCGTCTCA HGT14-6R TCCTCAACAGTCAAGTCCCTC HGT14-CF TCTAGCGAGCCGATTACAGT HGT14-CR CCCACATTGACCACCCAC H852 ATGTTGGCGACCTCGTATTGG H850 TTCCTCCCTTTATTTCAGATTCAA H855-R GCTGATCTGACCAGTTGC H856-F GTCGATGCGACGCAATCGT HGT14-qPCR-F GTGGGAGATATCGAGACGAAAC HGT14-qPCR-R ATCTGCCAGCCCTTGATTAC G6PDH-F TCAGAACAAGTTCGAGGGCGACAA G6PDH-R TGAGGGCAATAGAGGGCTTGTTCA

7 WP_ _Sinorhizobium_meliloti XP_ _Aspergillus_terreus WP_ _Bradyrhizobium_sp WP_ _Bradyrhizobium_sp WP_ _Methylobacterium_sp WP_ _Rhizobium_grahamii XP_ _Rhinocladiella_mackenziei WP_ _Bosea_thiooxidans KQR77015_Burkholderia_sp KGO54378_Penicillium_expansum XP_ _Pestalotiopsis_fici AIC2_Rhizobium_etli KIH762_Sporothrix_brasiliensis KEQ566_Aureobasidium_melanogenum XP_ _Exophiala_aquamarina WP_ _Bosea_thiooxidans XP_ _Aureobasidium_namibiae KFX361_Pseudogymnoascus_pannorum WP_ _Rhizobium_etli XP_ _Exophiala_xenobiotica KJR86105_Sporothrix_schenckii KKY16813_Phaeomoniella_chlamydospora WP_ _Bosea_sp KIW133_Exophiala_spinifera XP_ _Pseudocercospora_fijiensis WP_ _Elioraea_tepidiphila XP_ _Capronia_epimyces CRL21828_Penicillium_camemberti CEJ10414_bacterium_YEK KEQ90_Aureobasidium_pullulans VM1G_00056_Valsa_mali KIW34027_Cladophialophora_immunda XP_ _Talaromyces_stipitatus CDX52783_Mesorhizobium_plurifarium XP_ _Aureobasidium_subglaciale KQU83927_Mesorhizobium_sp CRG84517_Talaromyces_islandicus WP_ _Elioraea_tepidiphila GAM33785_Talaromyces_cellulolyticus XP_ _Fonsecaea_pedrosoi XP_ _Cyphellophora_europaea KRE00578_Bosea_sp EPE08060_Ophiostoma_piceae ERS919_Sporothrix_schenckii WP_ _Paenibacillus_odorifer GAD627_Byssochlamys_spectabilis KRE06713_Bosea_sp WP_ _Mesorhizobium_sp XP_ _Baudoinia_panamericana Bordetella_spp. Fusarium_spp. VM1G_00056

8 WP _Sphingobium_yanoikuyae EYE663_Aspergillus_ruber WP _Kutzneria_albida XP _Colletotrichum_fioriniae KKO503_Trichoderma_harzianum WP _Terracidiphilus_gabretensis KMS54549_Sphingobium_czechense WP _Sphingobium_sp WP _Streptomyces_sp WP _Caulobacter_henricii WP _Sphingomonas_sp CAK41643_Aspergillus_niger WP _Sphingomonas_sp WP _Novosphingobium_malaysiense WP018364_Streptomyces_sp XP _Bipolaris_sorokiniana XP _Zymoseptoria_tritici KOC10800_Aspergillus_flavus WP _Sphingobium_chungbukense WP _Novosphingobium_sp XP _Setosphaeria_turcica WP _Caulobacter_henricii WP _Caulobacter_sp WP _Sphingobium_sp EWM18849_Kutzneria_sp GAA86910_Aspergillus_kawachii EAU69263_Stigmatella_aurantiaca XP _Aspergillus_niger KKY241_Diaporthe_ampelina GAM05656_Novosphingobium_sp WP _Sphingomonas_sp KFY42778_Pseudogymnoascus_sp WP _Granulicella_mallensis WP _Sphingobium_japonicum WP _Asticcacaulis_biprosthecium XP _Bipolaris_zeicola WP _Shewanella_sp CCT70624_Fusarium_fujikuroi XP _Pantholops_hodgsonii GAP82757_Rosellinia_necatrix WP _Sphingobium_yanoikuyae CEJ83726_Torrubiella_hemipterigena WP _Rhodanobacter_sp XP _Fusarium_pseudograminearum XP00859_Beauveria_bassiana WP _Rhodanobacter_sp WP _Stigmatella_aurantiaca WP _Actinoplanes_friuliensis XP _Colletotrichum_gloeosporioides KLO763_Fusarium_fujikuroi EZP83146_Novosphingobium_resinovorum WP _Caulobacter_sp EHA27409_Aspergillus_niger WP _Rhizobacter_sp KNG444_Stemphylium_lycopersici KJK67468_Aspergillus_parasiticus WP _Kutzneria_albida WP _Novosphingobium_sp KMS50525_Novosphingobium_barchaimii EMF10247_Sphaerulina_musiva ABZ728_Caulobacter_sp WP _Pedobacter_sp XP681701_Aspergillus_nidulans WP _Rhodanobacter_fulvus WP _Kutzneria_sp VM1G_00616_Valsa_mali XP _Bipolaris_oryzae KEQ82843_Aureobasidium_pullulans XP _Pestalotiopsis_fici KGQ057_Beauveria_bassiana AEU37208_Granulicella_mallensis WP _Novosphingobium_sp KJX524_Zymoseptoria_brevis WP _Sphingobium_yanoikuyae WP _Microbispora_rosea WP _Sphingobium_sp WP _Novosphingobium_lindaniclasticum WP _Methylibium_sp WP _Amycolatopsis_balhimycina XP _Pyrenophora_tritici WP _Sphingomonas_sp WP _Caulobacter_sp WP _Caulobacter_sp WP _Novosphingobium_resinovorum XP _Aspergillus_flavus XP _Aureobasidium_subglaciale WP _Sphingomonas-like KEQ67483_Aureobasidium_melanogenum WP _Novosphingobium_sp WP _Sphingobium_yanoikuyae EQB52649_Colletotrichum_gloeosporioides WP _alpha_proteobacterium XP _Marssonina_brunnea KIS30550_Novosphingobium_sp XP _Pyrenophora_teres WP _Caulobacter_sp XP _Parastagonospora_nodorum XP _Aureobasidium_namibiae XP _Bipolaris_victoriae WP _Hassallia_byssoidea XP _Fusarium_graminearum VM1G_00616

9 KKY35092_Diaporthe_ampelina WP_01111_Rhodopirellula_baltica WP_ _Paenibacillus_sp WP_ _Verrucomicrobium_sp WP_ _Rhodopirellula_baltica WP_ _Rubinisphaera_brasiliensis KLU07046_Rhodopirellula_sp WP_ _Gimesia_maris WP_00308_Rhodopirellula_baltica WP_ _Haloferula_sp XP_ _Naegleria_gruberi WP_00228_Paenibacillus_sp WP_ _Rhodopirellula_sp WP_ _Verrucomicrobium_sp WP_ _Pirellula_staleyi EMI25847_Rhodopirellula_europaea WP_ _Pedosphaera_parvula WP_00913_Verrucomicrobium_spinosum EPX57826_Cystobacter_fuscus WP_ _Rhodopirellula_sp WP_ _Paenibacillus_lactis WP_ _Chthoniobacter_flavus WP_ _Zavarzinella_formosa WP_ _Cystobacter_fuscus WP_ _Rhodopirellula_maiorica WP_ _Verrucomicrobium_sp ADG68792_Planctopirus_limnophila WP_ _Rhodopirellula_baltica ADB15537_Pirellula_staleyi WP_ _Paenibacillus_sp WP_ _Singulisphaera_acidiphila WP_ _Paenibacillus_sp VM1G_00776_Valsa_mali WP_ _Planctopirus_limnophila WP_ _Rhodopirellula_europaea WP_ _Paenibacillus_sp WP_ _Verrucomicrobium_sp WP_ _Blastopirellula_marina WP_ _Rhodopirellula_europaea M1G_00776

10 EKX68546_Streptomyces_ipomoeae XP_ _Eutypa_lata VM1G_01172_Valsa_mali AAA67510_Streptomyces_glaucescens AGP609_Streptomyces_rapamycinicus KIM364_Oidiodendron_maius WP_ _Streptomyces_sp KIM9_Oidiodendron_maius WP_ _Streptomyces_caatingaensis WP_ _Lechevalieria_aerocolonigenes KJK50809_Lechevalieria_aerocolonigenes WP_ _Streptomyces_iranensis CEL20688_Kibdelosporangium_sp WP_ _Streptomyces_caatingaensis CDM28614_Penicillium_roqueforti WP_ _Herbidospora_cretacea WP_ _Pseudonocardia_acaciae WP_ _Allokutzneria_albata WP_00403_Saccharopolyspora_erythraea AFU90406_Streptomyces_sp WP_ _Streptomyces_bottropensis WP_ _Streptomyces_ipomoeae WP_ _Streptomyces_sp WP_ _Allokutzneria_albata WP_01870_Streptomyces_purpureus WP_ _Streptomyces_violaceusniger WP_ _Streptomyces_sp XP_ _Pestalotiopsis_fici WP_ _Streptomyces_himastatinicus WP_ _Mycobacterium_llatzerense WP_ _Streptomyces_mobaraensis WP_ _Streptomyces_sp WP_ _Amycolatopsis_rifamycinica AFV25479_Streptomyces_sp WP_01436_Streptomyces_sulphureus WP_ _Kibdelosporangium_phytohabitans WP_ _Streptomyces_scabiei WP_ _Archangium_gephyra WP_ _Streptomyces_hygroscopicus Mycobacterium_spp Mycobacterium_spp VM1G_01172

11 WP_ _Novosphingobium_malaysiense WP_ _Sphingomonas_sp KOP40348_Madurella_mycetomatis XP_ _Eutypa_lata WP_ _Sphingomonas_phyllosphaerae WP_01681_Sphingomonas_sp XP_ _Coniosporium_apollinis WP_ _Sphingomonas-like WP_ _Sphingomonas_sp KMS52368_Sphingobium_czechense WP_ _Novosphingobium_sp WP_ _Sphingomonas_sp KKY366_Diaporthe_ampelina WP_ _Sphingopyxis_alaskensis WP_ _Novosphingobium_resinovorum WP_ _Sphingomonas_paucimobilis WP_ _Novosphingobium_sp XP_ _Togninia_minima WP_ _Sphingomonas_sp KFA55220_Stachybotrys_chartarum WP_ _Sphingomonadaceae XP_ _Microsporum_gypseum WP_ _Sphingomonas_sp XP_ _Aureobasidium_subglaciale KMS54367_Novosphingobium_barchaimii WP_ _Sphingomonas_paucimobilis WP_ _Sphingomonas_sp WP_ _Sphingopyxis_sp WP_ _Sphingobium_sp WP_ _Sphingomonas_sp WP_04385_Novosphingobium_sp WP_ _Novosphingobium_sp EKG044_Macrophomina_phaseolina WP_ _Novosphingobium_sp WP_ _Novosphingobium_sp WP_ _Sphingomonadaceae WP_ _Novosphingobium_sp WP_ _Novosphingobium_lindaniclasticum WP_ _Novosphingobium_sp WP_ _Sphingomonas_sp KEQ619_Aureobasidium_melanogenum WP_ _Tolypothrix_campylonemoides VM1G_01377_Valsa_mali XP_ _Capronia_epimyces Sphingomonas_spp. Fusarium_spp. Pseudogymnoascus_spp. VM1G_01377

12 WP_ _Micromonospora_sp WP_ _Candidatus_Solibacter KRB77082_Nocardioides_sp WP_ _Granulicella_mallensis WP_ _Nocardiopsis_alba WP_ _bacterium_UASB WP_ _Acidobacteriaceae_bacterium KKY217_Diaporthe_ampelina WP_ _Nocardiopsis_baichengensis WP_ _Terriglobus_sp WP_ _Acidobacteriaceae_bacterium WP_ _Nocardiopsis_alba WP_ _Nocardiopsis_potens WP_ _Micromonospora_parva WP_ _Candidatus_Koribacter XP_ _Nectria_haematococca WP_ _Acidobacterium_sp KQP78010_Aeromicrobium_sp WP_ _Acidobacteria_bacterium WP_ _Edaphobacter_aggregans WP_ _Kribbella_flavida WP_ _Acidobacteriaceae_bacterium ACO33744_Acidobacterium_capsulatum WP_ _Acidobacteriaceae_bacterium WP_ _Granulicella_tundricola KQP26891_Aeromicrobium_sp WP_ _Acidobacteriaceae_bacterium VM1G_01892_Valsa_mali WP_ _Nocardiopsis_halophila WP_ _Acidobacteriaceae_bacterium XP_ _Pestalotiopsis_fici WP_ _Acidobacterium_capsulatum WP_ _Terriglobus_roseus WP_ _Nocardiopsis_sp WP_ _Nocardiopsis_chromatogenes Streptomyces_spp VM1G_01892

13 KJJ21279_Penicillium_solitum XP_ _Agaricus_bisporus VM1G_02284_Valsa_mali KIY62258_Cylindrobasidium_torrendii XP_ _Rhinocladiella_mackenziei CAI43283_Malassezia_sympodialis XP_ _Auricularia_subglabra KGO75825_Penicillium_italicum KIJ090_Laccaria_amethystina CEH18770_Ceraceosorus_bombacis EEB884_Moniliophthora_perniciosa KIW63858_Capronia_semiimmersa CEJ58114_Penicillium_brasilianum EKV115_Penicillium_digitatum XP_ _Moniliophthora_roreri XP_00260_Agaricus_bisporus CRG84226_Talaromyces_islandicus XP_ _Auricularia_subglabra XP_00272_Agaricus_bisporus KOS16334_Malassezia_pachydermatis XP_ _Anthracocystis_flocculosa KOS16335_Malassezia_pachydermatis KPV76585_Rhodotorula_graminis XP_ _Tilletiaria_anomala XP_ _Auricularia_subglabra XP_ _Capronia_coronata XP_ _Moniliophthora_roreri XP_ _Malassezia_globosa KIW34086_Cladophialophora_immunda CDI54338_Melanopsichium_pennsylvanicum KGO420_Penicillium_expansum KIW42617_Exophiala_oligosperma CDI554_Melanopsichium_pennsylvanicum XP_ _Schizophyllum_commune XP_ _Ustilago_maydis EMS260_Rhodosporidium_toruloides ETS65339_Pseudozyma_aphidis GAK65507_Pseudozyma_antarctica CBQ6_Sporisorium_reilianum GAK684_Pseudozyma_antarctica XP_ _Exophiala_xenobiotica KIY44082_Fistulina_hepatica CDM35827_Penicillium_roqueforti XP_ _Malassezia_globosa XP_ _Schizophyllum_commune CCF50110_Ustilago_hordei GAC024_Pseudozyma_antarctica KKY36776_Diaporthe_ampelina ETS61786_Pseudozyma_aphidis GAD92055_Byssochlamys_spectabilis KIY70183_Cylindrobasidium_torrendii KIW17623_Exophiala_spinifera XP_ _Pseudozyma_hubeiensis XP_ _Malassezia_globosa XP_ _Pseudozyma_hubeiensis EST09215_Pseudozyma_brasiliensis GAC71446_Pseudozyma_antarctica XP_ _Anthracocystis_flocculosa KIK66615_Gymnopus_luxurians EGU12718_Rhodotorula_glutinis XP_ _Talaromyces_marneffei KDQ31492_Pleurotus_ostreatus GAM40240_Talaromyces_cellulolyticus XP_ _Penicillium_rubens KIY01127_Fonsecaea_multimorphosa KOS37086_Penicillium_nordicum XP_ _Exophiala_dermatitidis CRL26481_Penicillium_camemberti KIV78046_Exophiala_sideris XP_ _Capronia_epimyces XP_ _Talaromyces_stipitatus Basidiomycota Basidiomycota Ascomycota VM1G_02284

14 WP_ _Streptomyces_sp GAM366_Talaromyces_cellulolyticus KOS44646_Penicillium_nordicum XP_ _Rhinocladiella_mackenziei XP_ _Cladophialophora_carrionii CCD565_Botrytis_cinerea KGO68501_Penicillium_italicum KIW350_Cladophialophora_bantiana XP_ _Eutypa_lata WP_ _Vibrio_nigripulchritudo GAM832_fungal_sp WP_ _Saccharospirillum_impatiens KMO772_Mycobacterium_obuense XP_ _Colletotrichum_fioriniae XP_ _Cladophialophora_yegresii EPE030_Ophiostoma_piceae XP_ _Marssonina_brunnea KIW024_Capronia_semiimmersa CEJ516_Penicillium_brasilianum XP_ _Botrytis_cinerea KIW16780_Exophiala_spinifera KQY06207_Mycobacterium_sp CDK28010_Kuraishia_capsulata KKY27433_Diplodia_seriata KEY74755_Stachybotrys_chartarum KKP037_Trichoderma_harzianum WP_ _Streptomyces_griseoflavus KIX3_Fonsecaea_multimorphosa KQR7_Williamsia_sp XP_ _Capronia_coronata KIW34586_Cladophialophora_immunda KIW00318_Verruconis_gallopava ETW9_Candidatus_Entotheonella XP_ _Sclerotinia_sclerotiorum KIV82153_Exophiala_sideris KFY76565_Pseudogymnoascus_pannorum CRG85410_Talaromyces_islandicus CDM27871_Penicillium_roqueforti WP_ _Streptomyces_sp KGO40120_Penicillium_expansum KFZ104_Pseudogymnoascus_pannorum VM1G_02549_Valsa_mali XP_ _Pestalotiopsis_fici WP_ _Limnohabitans_sp CRL18751_Penicillium_camemberti XP_ _Grosmannia_clavigera XP_ _Coniosporium_apollinis WP_ _Mycobacterium_sp XP_ _Rasamsonia_emersonii KJJ10128_Penicillium_solitum WP_ _Chelativorans_sp KPI35356_Phialophora_attae KKY18201_Phaeomoniella_chlamydospora XP_ _Talaromyces_marneffei KIW880_Cladophialophora_bantiana KFX42121_Talaromyces_marneffei WP_ _Mycobacterium_obuense XP_ _Cladophialophora_psammophila ETR7_Trichoderma_reesei XP_ _Penicillium_rubens XP_ _Fonsecaea_pedrosoi WP_ _Mycobacterium_obuense XP_ _Exophiala_xenobiotica XP_ _Fonsecaea_pedrosoi EMR87546_Botrytis_cinerea WP_ _Mycobacterium_vulneris ESZ586_Sclerotinia_borealis XP_ _Togninia_minima XP_ _Eutypa_lata KPI424_Phialophora_attae KFA45846_Stachybotrys_chartarum WP_ _Streptomyces_toyocaensis Bordetella_spp. Acinetobacter_spp. VM1G_02549

15 KIW16782_Exophiala_spinifera KEC78234_Sinorhizobium_americanum WP_ _Bordetella_bronchiseptica WP_03723_Rhodococcus_wratislaviensis WP_ _Streptomyces_sp KIW00312_Verruconis_gallopava WP_ _Bordetella_bronchiseptica XP_ _Neofusicoccum_parvum XP_00702_Pseudocercospora_fijiensis WP_ _Burkholderia_mimosarum WP_ _Bacillus_sp KFX42120_Talaromyces_marneffei WP_ _Sodalis_praecaptivus XP_ _Rhinocladiella_mackenziei WP_ _Acinetobacter_sp WP_ _Novosphingobium_malaysiense WP_ _Streptomyces_scabiei WP_ _Vibrio_sp WP_ _Streptomyces_sp WP_ _Rhodococcus_sp WP_ _Mycobacterium_vaccae WP_ _Bacillus_bataviensis AAW28146_Rhizobium_sp XP_ _Togninia_minima WP_ _Streptomyces_sp WP_ _Streptomyces_griseus WP_ _Acinetobacter_brisouii WP_ _Novosphingobium_resinovorum GAM368_Talaromyces_cellulolyticus EPE023_Ophiostoma_piceae WP_ _Streptomyces_xylophagus WP_ _Streptomyces_ipomoeae WP_ _Sodalis_glossinidius WP_ _Erwinia_typographi WP_ _Arthrobacter_sp WP_ _Sinorhizobium_americanum WP_ _Nocardia_higoensis WP_ _Klebsiella_pneumoniae WP_ _Bordetella_bronchiseptica WP_ _Bradyrhizobium_sp XP_ _Pestalotiopsis_fici WP_ _Pimelobacter_simplex KKO72846_Kerstersia_gyiorum KEY74761_Stachybotrys_chartarum XP_ _Talaromyces_marneffei WP_ _Mycobacterium_sp WP_ _Ensifer_sp WP_ _Hyphomicrobium_sp WP_ _Streptomyces_phaeochromogenes XP_ _Capronia_coronata WP_ _Streptomyces_acidiscabies VM1G_02555_Valsa_mali KFA81538_Stachybotrys_chartarum WP_ _Raoultella_terrigena EGD06287_Burkholderia_sp WP_ _Bordetella_bronchiseptica WP_ _Pusillimonas_noertemannii KKY16861_Phaeomoniella_chlamydospora WP_ _Streptomyces_sp XP_ _Eutypa_lata WP_ _Bordetella_parapertussis WP_ _Bacillus_sp WP_ _Streptomyces_acidiscabies WP_ _Bacillus_sp WP_ _Nocardioides_alkalitolerans WP_ _Amycolatopsis_taiwanensis KPI35307_Phialophora_attae XP_ _Grosmannia_clavigera WP_ _Rhodococcus_sp WP_ _Pusillimonas_noertemannii Achromobacter_spp Photorhabdus_spp VM1G_02555

16 KDQ13290_Botryobasidium_botryosum CEL57465_Rhizoctonia_solani KDQ58426_Jaapia_argillacea EUC53763_Rhizoctonia_solani KIO242_Tulasnella_calospora XP_ _Punctularia_strigosozonata KIO24292_Tulasnella_calospora XP_ _Gloeophyllum_trabeum VM1G_02910_Valsa_mali CCO26677_Rhizoctonia_solani EJU02432_Dacryopinax_sp XP_ _Auricularia_subglabra CUA77546_Rhizoctonia_solani KIO24291_Tulasnella_calospora XP_ _Fomitiporia_mediterranea KII87568_Plicaturopsis_crispa KLO122_Schizopora_paradoxa KIO24307_Tulasnella_calospora KLO13341_Schizopora_paradoxa XP_ _Pseudocercospora_fijiensis XP_ _Auricularia_subglabra KIO216_Tulasnella_calospora KIO26062_Tulasnella_calospora KIO24280_Tulasnella_calospora CED84805_Xanthophyllomyces_dendrorhous KIM123_Serendipita_vermifera CCA74032_Piriformospora_indica Cryptococcus_spp Basidiomycota Basidiomycota Ascomycota VM1G_02910

17 EYE023_Aspergillus_ruber KGO304_Penicillium_expansum KIV762_Exophiala_sideris EUN26561_Bipolaris_victoriae XP_ _Melampsora_larici XP_ _Pestalotiopsis_fici XP_ _Colletotrichum_gloeosporioides KGO75074_Penicillium_italicum KOS478_Penicillium_nordicum KDN66752_Colletotrichum_sublineola AF181866_synthetic_construct XP_456462_Debaryomyces_hansenii XP_00829_Punctularia_strigosozonata KIV86820_Exophiala_sideris XP_ _Aureobasidium_subglaciale XP_ _Talaromyces_stipitatus XP_ _Bipolaris_sorokiniana XP_ _Setosphaeria_turcica 4RSL_A_4RSL_A XP_ _Nectria_haematococca CEJ56755_Penicillium_brasilianum KGO47403_Penicillium_expansum XP_ _Talaromyces_stipitatus XP_ _Stereum_hirsutum KKY34247_Diaporthe_ampelina EQB58906_Colletotrichum_gloeosporioides BAD00185_Penicillium_terrenum XP_ _Aureobasidium_namibiae XP_ _Pyrenophora_teres CRL24868_Penicillium_camemberti EPE02569_Ophiostoma_piceae XP_ _Exophiala_xenobiotica KIW10542_Exophiala_spinifera XP_ _Nectria_haematococca XP_ _Grosmannia_clavigera XP_ _Exophiala_dermatitidis XP_ _Neofusicoccum_parvum BAE140_Ulocladium_sp XP_ _Bipolaris_maydis XP_ _Exophiala_xenobiotica KII88422_Plicaturopsis_crispa KIY458_Fistulina_hepatica XP_ _Bipolaris_zeicola ENH83077_Colletotrichum_orbiculare XP_681392_Aspergillus_nidulans XP_ _Eutypa_lata XP_ _Puccinia_graminis CDK252_Kuraishia_capsulata CDZ665_Xanthophyllomyces_dendrorhous CDM34716_Penicillium_roqueforti XP_ _Bipolaris_oryzae XP_ _Pyrenophora_tritici XP_777019_Cryptococcus_neoformans KNZ529_Puccinia_sorghi VM1G_020_Valsa_mali GAM35130_Talaromyces_cellulolyticus KNE892_Puccinia_striiformis XP_ _Parastagonospora_nodorum KEZ43556_Scedosporium_apiospermum XP_ _Heterobasidion_irregulare KDQ51628_Jaapia_argillacea XP_00255_Penicillium_rubens KEQ614_Aureobasidium_melanogenum XP_ _Capronia_coronata KJJ23348_Penicillium_solitum CEP22126_Cyberlindnera_jadinii XP_568763_Cryptococcus_neoformans KEQ80229_Aureobasidium_pullulans BAD00186_Coniochaeta_sp XP_ _Colletotrichum_graminicola XP_ _Cryptococcus_neoformans XP_ _Schizophyllum_commune XP_ _Gloeophyllum_trabeum XP_ _Wickerhamomyces_ciferrii Fusarium_spp Cryptococcus_spp Basidiomycota Ascomycota VM1G_020

18 WP_ _Burkholderia_sp AJC22888_Pandoraea_pulmonicola WP_ _Burkholderia_sp WP_ _Xenophilus_azovorans WP_ _Rhodococcus_sp KIM700_Oidiodendron_maius WP_ _Azohydromonas_australica WP_ _Burkholderia_xenovorans WP_ _Comamonas_testosteroni WP_ _Nocardia_jiangxiensis WP_ _Rhodococcus_sp WP_ _Streptomyces_europaeiscabiei KPI23374_Actinobacteria_bacterium KRB187_Hydrogenophaga_sp WP_ _Sciscionella_sp WP_ _Sphingobium_baderi WP_ _Bosea_sp KMS61908_Sphingobium_baderi WP_ _Amorphus_coralli KEY74811_Stachybotrys_chartarum KFA4_Stachybotrys_chartarum GAO20820_Alicycliphilus_sp WP_ _Ottowia_thiooxydans EIF318_Burkholderia_sp WP_ _Pandoraea_pulmonicola KRB85535_Sphingomonas_sp WP_ _Burkholderia_sp WP_ _Sphingopyxis_sp WP_ _Sciscionella_marina EYS85102_Cupriavidus_sp AJW201_Sphingomonas_sp XP_008823_Colletotrichum_graminicola XP_ _Setosphaeria_turcica WP_ _Massilia_sp WP_ _Burkholderia_sp WP_ _Burkholderia_multivorans KGO63929_Penicillium_italicum WP_ _Burkholderia_multivorans EIF32032_Burkholderia_sp WP_ _Cupriavidus_sp XP_ _Rhinocladiella_mackenziei WP_ _Rhodococcus_jostii VM1G_03090_Valsa_mali AKP92220_Achromobacter_xylosoxidans VM1G_03090

19 0.3 KQV30626_Rhizobium_sp WP_ _Arenibacter_algicola KFZ12530_Pseudogymnoascus_pannorum EWG42788_Fusarium_verticillioides KPA45902_Fusarium_langsethiae WP_ _Candidatus_Solibacter WP_01623_Eudoraea_adriatica WP_ _Acidobacteriaceae_bacterium KFY43621_Pseudogymnoascus_pannorum CRK16278_Verticillium_longisporum KFY710_Pseudogymnoascus_pannorum XP_ _Arthroderma_otae KKY31257_Diaporthe_ampelina WP_02615_Rhizobium_leguminosarum KIL912_Fusarium_avenaceum KFY30424_Pseudogymnoascus_pannorum WP_ _Flavobacterium_filum XP_ _Colletotrichum_fioriniae XP_ _Verticillium_dahliae EXM07238_Fusarium_oxysporum VM1G_03108_Valsa_mali WP_ _Lacinutrix_himadriensis WP_ _Arenitalea_lutea WP_ _Candidatus_Phaeomarinobacter WP_ _Xanthomonas_sp KFX86644_Pseudogymnoascus_pannorum XP_ _Fusarium_pseudograminearum XP_ _Verticillium_alfalfae ENH68826_Fusarium_oxysporum XP_ _Anthracocystis_flocculosa CRK14657_Verticillium_longisporum WP_ _Acidobacteriaceae_bacterium WP_ _Niabella_soli XP_ _Cyphellophora_europaea CRL17750_Penicillium_camemberti KFH68184_Mortierella_verticillata GAO88553_Neosartorya_udagawae WP_ _Corynebacterium_genitalium WP_ _Rhizobium_grahamii ALM086_Sediminicola_sp CCT66908_Fusarium_fujikuroi EMT68080_Fusarium_oxysporum XP_ _Pseudocercospora_fijiensis XP_ _Melampsora_larici EWZ727_Fusarium_oxysporum WP_ _Terriglobus_sp XP_ _Microsporum_gypseum WP_02903_Rhizobium_leguminosarum KFX2_Pseudogymnoascus_pannorum KLO632_Fusarium_fujikuroi XP_ _Fusarium_graminearum WP_ _Sphingobacterium_sp Chryseobacterium_spp VM1G_03108

20 XP _Parastagonospora_nodorum XP _Neosartorya_fischeri WP _Chryseobacterium_sp GAM319_Talaromyces_cellulolyticus GAD648_Byssochlamys_spectabilis CEJ62862_Penicillium_brasilianum WP _Lactobacillus_plantarum KIY01641_Fonsecaea_multimorphosa KIV86245_Exophiala_sideris KIV87176_Exophiala_sideris KJK64811_Aspergillus_parasiticus XP _Phaeoacremonium_minimum XP _Dichomitus_squalens WP _Frankia_sp WP _Nocardia_niigatensis GAQ11743_Aspergillus_lentulus CAJ62281_Frankia_alni XP00752_Eutypa_lata CRL172_Penicillium_camemberti XP _Colletotrichum_graminicola GAO858_Neosartorya_udagawae KFY420_Pseudogymnoascus_sp XP _Glarea_lozoyensis XP _Capronia_epimyces WP _Kutzneria_albida GAM35249_Talaromyces_cellulolyticus WP _Algoriphagus_sp EMR911_Botrytis_cinerea XP7449_Aspergillus_fumigatus XP _Bipolaris_maydis WP _Lactobacillus_silagei KJK63712_Aspergillus_parasiticus WP _WP_ WP _Nocardia_vinacea KLP06860_Fusarium_fujikuroi XP _Capronia_coronata KIV78870_Exophiala_sideris KKZ655_Emmonsia_crescens VM1G_03556_Valsa_mali KJJ25285_Penicillium_solitum WP04176_Frankia_alni XP _Rhinocladiella_mackenziei WP _Paenibacillus_sp XP _Capronia_coronata KIW012_Exophiala_spinifera CRL24591_Penicillium_camemberti XP _Botrytis_cinerea XP _Moniliophthora_roreri XP _Exophiala_xenobiotica KDN71869_Colletotrichum_sublineola XP _Talaromyces_stipitatus XP _Cyphellophora_europaea XP _Thielavia_terrestris KOS216_Escovopsis_weberi GAM43929_Talaromyces_cellulolyticus XP _Pestalotiopsis_fici KOS482_Penicillium_nordicum WP _Rhodococcus_rhodochrous XP _Cladophialophora_carrionii XP _Colletotrichum_fioriniae XP _Exophiala_dermatitidis XP _Aspergillus_flavus KJA23206_Hypholoma_sublateritium CRG83420_Talaromyces_islandicus WP _Sphingomonas_sp XP _Colletotrichum_gloeosporioides Ascomycota Ascomycota Basidiomycota VM1G_03556

21 WP_ _Gordonia_sputi WP_ _Microbispora_rosea VM1G_04133_Valsa_mali WP_ _Microbacterium_testaceum WP_ _Shinella_sp WP_02001_Mycobacterium_sp WP_04701_Nonomuraea_sp WP_02681_Mycobacterium_sp KQM34063_Rhizobium_sp WP_ _Micromonospora_chokoriensis WP_ _Microbacterium_hominis WP_ _Azorhizobium_doebereinerae WP_ _Gordonia_otitidis KQQ534_Rhizobium_sp WP_ _Nonomuraea_sp WP_ _Mycobacterium_sp ESY88146_Mesorhizobium_sp WP_ _Terriglobus_roseus WP_ _Mycobacterium_sp WP_ _Shinella_sp WP_ _Mesorhizobium_sp WP_ _Mycobacterium_llatzerense WP_ _Nocardia_higoensis WP_ _Nonomuraea_sp WP_ _Pedosphaera_parvula WP_ _Streptomyces_scabrisporus WP_ _Mycobacterium_sp WP_ _Mesorhizobium_sp XP_ _Baudoinia_panamericana VM1G_04133

22 WP_ _Rhizobium_leguminosarum XP_ _Nicotiana_sylvestris WP_ _Streptomyces_cellulosae WP_ _Paenibacillus_sp WP_ _Arthrobacter_sp XP_ _Nicotiana_tomentosiformis XP_ _Ogataea_parapolymorpha ABR16229_Picea_sitchensis XP_ _Erythranthe_guttatus AFZ84925_Physcomitrella_patens KCW88013_Eucalyptus_grandis WP_ _Runella_limosa KCW88014_Eucalyptus_grandis WP_ _Paenibacillus_peoriae CDK279_Kuraishia_capsulata WP_ _Photobacterium_sanctipauli EFL20921_Streptomyces_himastatinicus WP_ _Paenibacillus_senegalensis EIF45186_Brettanomyces_bruxellensis WP_ _Cylindrospermum_stagnale WP_ _Clostridiales_bacterium WP_ _Paenibacillus_sp XP_ _Solanum_lycopersicum WP_ _Corynebacterium-like WP_ _Bacillus_sp XP_ _Brachypodium_distachyon AFZ84926_Physcomitrella_patens WP_ _Devosia_sp XP_ _Togninia_minima BAJ480_Candidatus_Caldiarchaeum XP_ _Elaeis_guineensis WP_ _Streptomyces_violaceoruber KON30581_miscellaneous_Crenarchaeota WP_ _Eubacterium_sp WP_ _Halomonas_salina BAJ471_Candidatus_Caldiarchaeum WP_ _Clostridium_sp AFZ84924_Physcomitrella_patens WP_ _Paenibacillus_sp WP_ _Mesorhizobium_sp AFZ84927_Physcomitrella_patens XP_ _Physcomitrella_patens WP_ _Streptomyces_sp WP_ _Clostridiales_bacterium AFZ84928_Physcomitrella_patens KPN14136_Bacillus_sp VM1G_04190_Valsa_mali WP_ _Scytonema_hofmanni WP_ _Bacillus_sp XP_ _Candida_tenuis Viridiplantae Viridiplantae VM1G_04190

23 0.09 XP_ _Colletotrichum_graminicola XP_ _Pestalotiopsis_fici XP_ _Phytophthora_parasitica KKY13676_Diplodia_seriata ETM415_Phytophthora_parasitica XP_ _Phytophthora_parasitica ETL31091_Phytophthora_parasitica CEJ56904_Penicillium_brasilianum EKV05820_Penicillium_digitatum XP_664276_Aspergillus_nidulans XP_ _Phytophthora_infestans XP_ _Colletotrichum_gloeosporioides ETO70679_Phytophthora_parasitica KGO77832_Penicillium_italicum ETI48538_Phytophthora_parasitica KPM40080_Neonectria_ditissima CCX34009_Pyronema_omphalodes EME43478_Dothistroma_septosporum EKG17_Macrophomina_phaseolina KJX177_Zymoseptoria_brevis ENH83916_Colletotrichum_orbiculare XP_00140_Phytophthora_sojae EQB47601_Colletotrichum_gloeosporioides EMF12245_Sphaerulina_musiva XP_ _Neofusicoccum_parvum XP_ _Phytophthora_sojae XP_ _Penicillium_rubens ETL88722_Phytophthora_parasitica CRL22911_Penicillium_camemberti XP_ _Nectria_haematococca XP_ _Phytophthora_parasitica XP_ _Phytophthora_infestans XP_ _Melampsora_larici XP_ _Phytophthora_sojae XP_ _Phytophthora_infestans XP_ _Colletotrichum_fioriniae CCF36503_Colletotrichum_higginsianum KGO46541_Penicillium_expansum ETO306_Phytophthora_parasitica XP_ _Phytophthora_sojae XP_ _Pseudocercospora_fijiensis XP_ _Phytophthora_infestans KDN63372_Colletotrichum_sublineola VM1G_04692_Valsa_mali XP_ _Puccinia_graminis KJJ039_Penicillium_solitum ETI44692_Phytophthora_parasitica KOS396_Penicillium_nordicum Fusarium_spp Ascomycota Ascomycota Basidiomycota Oomycetes VM1G_04692

24 WP_ _Streptomyces_europaeiscabiei ELQ35678_Magnaporthe_oryzae WP_ _Streptomyces_galbus KPI324_Actinobacteria_bacterium XP_ _Togninia_minima WP_ _Clostridium_scatologenes WP_ _Rhodococcus_rhodnii XP_ _Eutypa_lata VM1G_04714_Valsa_mali WP_ _Streptomyces_sp WP_ _Micromonospora_carbonacea WP_ _Streptomyces_scabiei ELP70762_Streptomyces_turgidiscabies WP_ _Streptomyces_bottropensis WP_ _Verrucosispora_maris KLU857_Magnaporthiopsis_poae WP_ _Kibdelosporangium_aridum WP_ _Jiangella_alkaliphila GAP83078_Rosellinia_necatrix XP_ _Leptosphaeria_maculans XP_ _Magnaporthe_oryzae WP_ _Streptomyces_phaeochromogenes KRD202_Streptomyces_sp EKX634_Streptomyces_ipomoeae WP_ _Streptomyces_griseus WP_ _Streptomyces_turgidiscabies KKY35501_Diaporthe_ampelina WP_ _Streptomyces_bingchenggensis WP_ _Jiangella_alkaliphila WP_ _Jiangella_gansuensis WP_ _Streptomyces_sp WP_ _Herbidospora_cretacea WP_ _Actinoplanes_globisporus WP_ _Streptomyces_afghaniensis XP_ _Gaeumannomyces_graminis ELQ63839_Magnaporthe_oryzae WP_ _Jiangella_muralis ADI039_Streptomyces_bingchenggensis WP_ _Streptomyces_ipomoeae KPM46055_Neonectria_ditissima KFF211_Streptomyces_scabiei WP_ _Jiangella_muralis WP_ _Jiangella_muralis WP_ _Actinospica_robiniae WP_ _Clostridium_drakei WP_ _Streptomyces_sp WP_ _Streptomyces_sp EPJ42815_Streptomyces_afghaniensis WP_03331_Streptomyces_yerevanensis WP_ _Streptomyces_griseoruber VM1G_04714

25 KFY72129_Pseudogymnoascus_pannorum WP_ _Pseudomonas_sp WP_ _Pseudomonas_sp WP_ _Agrobacterium_rhizogenes GAD0_Byssochlamys_spectabilis EQB50603_Colletotrichum_gloeosporioides EXK762_Fusarium_oxysporum XP_ _Nectria_haematococca VM1G_05155_Valsa_mali XP_ _Nectria_haematococca XP_ _Colletotrichum_gloeosporioides KFY12076_Pseudogymnoascus_pannorum WP_ _Caulobacter_segnis WP_ _Acinetobacter_baumannii KGO69138_Penicillium_expansum WP_ _Paenibacillus_sp KFY05889_Pseudogymnoascus_pannorum XP_ _Neofusicoccum_parvum WP_ _Agrobacterium_vitis KIL86485_Fusarium_avenaceum WP_ _Pseudomonas_fluorescens XP_ _Rasamsonia_emersonii WP_ _Serratia_sp WP_ _Rhodococcus_sp WP_ _Rhizobium_leguminosarum KFZ05818_Pseudogymnoascus_pannorum WP_ _Pseudomonas_brassicacearum EXL915_Fusarium_oxysporum XP_ _Colletotrichum_fioriniae WP_ _Paenibacillus_sp KGO47902_Penicillium_expansum XP_ _Nectria_haematococca KGO617_Penicillium_expansum Ascomycota Ascomycota VM1G_05155

26 0.3 WP_ _Bacillus_thuringiensis VM1G_05405_Valsa_mali EGV00186_Mycoplasma_columbinum KIR032_Lachnospiraceae_bacterium EMR90169_Botrytis_cinerea XP_ _Sclerotinia_sclerotiorum ESZ91503_Sclerotinia_borealis EWC47146_Drechslerella_stenobrocha WP_04660_Bacillus_cereus XP_ _Tuber_melanosporum AET42542_Emiliania_huxleyi WP_ _Mycoplasma_hyopneumoniae WP_048722_Bacillus_cytotoxicus CNS05861_Salmonella_enterica AET610_Emiliania_huxleyi CCX04588_Pyronema_omphalodes WP_ _Mycoplasma_bovis XP_ _Micromonas_pusilla WP_ _Bacillus_cereus WP_ _Mycoplasma_anatis WP_ _Ruminococcus_albus WP_ _Mycoplasma_agalactiae WP_ _Bacillus_cereus AKU788_Spiroplasma_turonicum KKY246_Diaporthe_ampelina WP_ _Bacillus_cereus KPU55275_Bacillus_cereus CDC49071_Clostridium_sp WP_ _Spiroplasma_apis CCA68150_Piriformospora_indica WP_01782_Ruminococcus_flavefaciens EGS282_Mycoplasma_anatis WP_ _WP_ WP_ _Bacillus_cereus WP_ _Mycoplasma_columbinum WP_ _Mycoplasma_columbinum WP_ _Mycoplasma_agalactiae WP_02338_Mycoplasma_glycophilum WP_ _Ruminococcus_albus WP_ _Mycoplasma_hyopneumoniae WP_ _Bacillus_thuringiensis WP_ _Bacillus_cereus XP_ _Botrytis_cinerea XP_ _Dactylellina_haptotyla CCD50810_Botrytis_cinerea WP_ _Mycoplasma_hyopneumoniae CCD50809_Botrytis_cinerea WP_ _Spiroplasma_syrphidicola WP_ _Bacillus_cereus YP_ _Cafeteria_roenbergensis AKA50185_Mycoplasma_gallinaceum WP_ _Mycoplasma_flocculare XP_ _Arthrobotrys_oligospora WP_ _Spiroplasma_apis KPI390_Phialophora_attae WP_ _Bacillus_cereus Ascomycota VM1G_05405

27 VM1G_05720 WP_ _Streptomycetaceae_bacterium 85 WP_ _Streptomyces_vitaminophilus WP_ _Streptomyces_peruviensis 83 WP_ _Streptomyces_viridochromogenes 74 WP_ _Streptomyces_viridochromogenes 67 WP_ _Streptomyces_aureofaciens WP_ _Streptomyces_scabrisporus 80 WP_ _Nonomuraea_coxensis KRF34641_Nocardioides_sp KRE443_Nocardioides_sp 84 KGO636_Penicillium_italicum KJJ26316_Penicillium_solitum 42 KGO43380_Penicillium_expansum KGO5_Penicillium_expansum 64 KIN08901_Oidiodendron_maius 75 VM1G_05720_Valsa_mali XP_ _Aureobasidium_namibiae GAM913_fungal_sp WP_01291_Kribbella_flavida WP_ _Kribbella_catacumbae WP_ _WP_

28 WP_ _Marinobacter_nanhaiticus KPV61707_Bathyarchaeota_archaeon ESN65425_Serratia_sp EKG16230_Macrophomina_phaseolina WP_ _Thermoanaerobacter_italicus WP_ _Clostridium_sp WP_ _Bifidobacterium_reuteri ADI02052_Syntrophothermus_lipocalidus WP_ _Clostridium_sp WP_ _Stigmatella_aurantiaca WP_ _Syntrophothermus_lipocalidus WP_ _Clostridium_sp WP_ _Clostridium_sp XP_ _Pestalotiopsis_fici EAU64700_Stigmatella_aurantiaca WP_ _Thermoanaerobacter_sp WP_ _Thermoanaerobacter_ethanolicus WP_ _Brenneria_goodwinii WP_ _Thermoanaerobacter_sp WP_ _Frankia_sp WP_ _Marinobacterium_litorale KFA45684_Stachybotrys_chartarum WP_ _Thermoanaerobacter_wiegelii WP_ _Clostridium_sp WP_ _Serratia_sp WP_ _Butyrivibrio_crossotus XP_ _Grosmannia_clavigera KEY71523_Stachybotrys_chartarum XP_ _Togninia_minima WP_ _Clostridium_sp WP_ _Clostridiaceae_bacterium KFI83206_Bifidobacterium_reuteri WP_ _Frankia_sp XP_ _Neofusicoccum_parvum KFA6_Stachybotrys_chlorohalonata WP_ _Pseudomonas_sp KFA71331_Stachybotrys_chartarum WP_ _Desulfotomaculum_thermocisternum VM1G_05918_Valsa_mali WP_ _Thermoanaerobacter_sp WP_ _Clostridium_argentinense WP_ _Caldanaerobacter_subterraneus CCY766_Butyrivibrio_crossotus KKY26283_Phaeomoniella_chlamydospora XP_00713_Capronia_epimyces WP_ _Acetivibrio_cellulolyticus WP_ _Desulfotomaculum_alkaliphilum WP_ _Ruminiclostridium_thermocellum WP_ _Clostridiales_bacterium VM1G_05918

29 KPI44658_Phialophora_attae XP _Cyphellophora_europaea VM1G_07081_Valsa_mali KIW13928_Exophiala_spinifera CCF657_Nocardia_cyriacigeorgica ADI11806_Streptomyces_bingchenggensis WP _Amycolatopsis_jejuensis WP03192_Nocardia_otitidiscaviarum XP _Talaromyces_stipitatus WP _Nocardia_farcinica WP _Mycobacterium_mageritense WP _Nocardia_cyriacigeorgica XP _Trichoderma_atroviride XP _Coccidioides_immitis EJZ13104_Mycobacterium_fortuitum CRZ15879_Mycobacterium_neworleansense WP _Mycobacterium_sp KMU87049_Coccidioides_immitis CRG873_Talaromyces_islandicus WP _Nocardia_cyriacigeorgica XP _Exophiala_aquamarina XP _Coccidioides_posadasii WP _Rhodococcus_erythropolis CEO59207_Penicillium_brasilianum WP _Streptomyces_bingchenggensis Fusarium_spp Mycobacterium_spp Mycobacterium_spp VM1G_07081

30 WP_ _Prevotella_veroralis WP_ _Methyloversatilis_universalis WP_ _Mesorhizobium_sp WP_ _Sphingopyxis_sp WP_ _Prevotella_melaninogenica WP_ _Pelomonas_sp WP_ _Anaerolinea_thermophila WP_ _Sphingomonas_sp WP_ _Nocardioides_sp WP_ _Prevotella_sp WP_04854_Tetrasphaera_japonica ABQ71016_Sphingomonas_wittichii WP_ _Mycobacterium_vaccae WP_ _Prevotella_multiformis CDA82828_Bacteroides_sp WP_ _Frigoribacterium_sp WP_ _Pseudomonas_fluorescens WP_ _Nocardioides_sp WP_ _Bacteroides_sp WP_ _Nocardioides_sp WP_ _Prevotella_nanceiensis WP_ _Georgenia_sp CRK15713_Verticillium_longisporum WP_02782_Anaerobiospirillum_succiniciproducens WP_ _Prevotella_sp EFC729_Prevotella_melaninogenica WP_ _Pseudomonas_sp WP_ _Methylocystis_sp WP_ _Sphingomonas_sp VM1G_088_Valsa_mali WP_ _Mycobacterium_goodii WP_ _Methyloversatilis_universalis WP_ _Frigoribacterium_sp WP_ _Sphingopyxis_macrogoltabida WP_ _Hyphomicrobium_zavarzinii WP_ _Curtobacterium_sp WP_ _Prevotella_sp WP_ _Prevotella_veroralis WP_ _Alkanindiges_illinoisensis WP_ _Phycicoccus_jejuensis WP_ _Bacteroides_sp WP_ _Mobilicoccus_sp KOR33108_Achromatium_sp CUO758_Bacteroides_xylanisolvens WP_ _Bacteroides_xylanisolvens WP_ _Pseudomonas_tuomuerensis WP_ _Mobilicoccus_pelagius WP_ _Pseudomonas_fluorescens WP_ _Candidatus_Achromatium AIV40612_Curtobacterium_sp WP_ _Pseudomonas_chlororaphis WP_ _Roseibacterium_elongatum WP_05641_Sphingomonas_sp VM1G_088

31 WP_ _Rubinisphaera_brasiliensis WP_ _Turicibacter_sanguinis WP_01134_Hahella_chejuensis KGZ109_Raoultella_planticola KPL13614_Bacteroides_sp WP_ _Gimesia_maris WP_ _Blastopirellula_marina WP_ _Methylosarcina_fibrata WP_ _Turicibacter_sanguinis KIW16080_Exophiala_spinifera WP_ _Helicobacter_ailurogastricus WP_ _Streptomyces_neyagawaensis AIT56388_uncultured_bacterium XP_ _Rhinocladiella_mackenziei WP_ _Streptomyces_mirabilis ADB40010_Spirosoma_linguale WP_ _Raoultella_planticola WP_ _Frankia_sp WP_ _Frankia_sp WP_ _Actinoplanes_globisporus CRF52922_Helicobacter_ailurogastricus XP_ _Capronia_coronata WP_ _Frankia_sp WP_ _Amycolatopsis_halophila XP_ _Exophiala_dermatitidis WP_ _Frankia_sp WP_ _Nocardiopsis_potens KIV80839_Exophiala_sideris WP_ _Turicibacter_sp WP_ _Streptomyces_europaeiscabiei KJE22112_Frankia_sp ADO74692_Stigmatella_aurantiaca WP_ _Streptomyces_bottropensis WP_ _Raoultella_sp KFD06387_Raoultella_planticola VM1G_09114_Valsa_mali WP_ _Stigmatella_aurantiaca KKY36417_Diaporthe_ampelina WP_ _Porphyrobacter_sp KQM04280_Frankia_sp WP_ _Caloramator_sp WP_ _Turicibacter_sanguinis VM1G_09114

32 0.1 WP_ _Rhodopseudomonas_sp WP_ _Pandoraea_sp WP_ _Burkholderia_contaminans WP_ _Rhodopseudomonas_sp WP_ _Burkholderiaceae_bacterium WP_ _Pandoraea_sp WP_ _Burkholderia_bannensis VM1G_10126_Valsa_mali WP_ _Cupriavidus_basilensis WP_ _Rhodopseudomonas_palustris WP_ _Cupriavidus_sp WP_040_Rhodopseudomonas_palustris AHB77920_Pandoraea_sp WP_ _Burkholderia_mimosarum WP_ _Burkholderia_phymatum WP_01129_Cupriavidus_pinatubonensis WP_ _Rhodopseudomonas_palustris WP_ _Burkholderia_sp WP_ _Rhodopseudomonas_palustris WP_02539_Pandoraea_pnomenusa WP_ _Pseudomonas_sp AHN788_Pandoraea_pnomenusa VM1G_10126

33 WP_ _Lampropedia_sp KKY15566_Phaeomoniella_chlamydospora XP_ _Bipolaris_oryzae ESW512_Rhodobacter_sp WP_ _Ochrobactrum_sp CDE50821_Faecalibacterium_sp KKY13883_Diplodia_seriata WP_ _unclassified_Clostridiales XP_ _Exophiala_xenobiotica WP_ _Ochrobactrum_intermedium CCD47517_Botrytis_cinerea WP_ _Lampropedia_sp XP_ _Bipolaris_zeicola ABP50426_Pyrobaculum_arsenaticum ABO08726_Pyrobaculum_calidifontis WP_047510_Rhizobium_sp XP_ _Notothenia_coriiceps WP_ _Rhizobium_sp BAN8_Aeropyrum_camini AFA320_Pyrobaculum_oguniense XP_ _Notothenia_coriiceps WP_ _Nitrospina_gracilis WP_ _WP_ XP_ _Capronia_epimyces WP_ _Caldivirga_maquilingensis XP_ _Cladophialophora_psammophila WP_ _Desulfurococcus_mucosus ESZ587_Sclerotinia_borealis WP_ _Rhizobium_sp XP_ _Maylandia_zebra XP_ _Fonsecaea_pedrosoi CDX58527_Mesorhizobium_plurifarium WP_ _Ochrobactrum_anthropi WP_ _Thermoproteus_tenax WP_ _Rhizobium_alamii KIW71727_Capronia_semiimmersa XP_ _Salmo_salar XP_00761_Bipolaris_sorokiniana BAA79068_Aeropyrum_pernix WP_ _Pyrobaculum_islandicum XP_ _Selaginella_moellendorffii WP_ _Pyrodictium_delaneyi KIV85086_Exophiala_sideris ACB407_Pyrobaculum_neutrophilum WP_ _Rhizobium_alamii KIX314_Fonsecaea_multimorphosa EEQ130_Ochrobactrum_intermedium VM1G_10361_Valsa_mali WP_ _Ochrobactrum_anthropi XP_ _Branchiostoma_floridae KNG44746_Stemphylium_lycopersici WP_ _Martelella_endophytica XP_ _Capronia_coronata AAL64263_Pyrobaculum_aerophilum WP_ _Pyrolobus_fumarii WP_ _Ochrobactrum_sp KJS17081_Hoeflea_sp WP_ _Ochrobactrum_intermedium XP_ _Cladophialophora_carrionii WP_ _Rhizobium_leguminosarum XP_ _Papilio_polytes CDK24869_Kuraishia_capsulata ERM02390_Ochrobactrum_intermedium XP_ _Aplysia_californica XP_ _Ogataea_parapolymorpha WP_ _Ochrobactrum_intermedium WP_ _Alphaproteobacteria WP_ _Ochrobactrum_intermedium WP_ _Ochrobactrum_sp WP_ _Desulfurococcus_mobilis WP_ _Arthrobacter_sp AKT35671_Pyrobaculum_sp KIW464_Exophiala_oligosperma EUN231_Bipolaris_victoriae KIW368_Cladophialophora_bantiana XP_ _Rhinocladiella_mackenziei KJS17077_Hoeflea_sp KIY45135_Fistulina_hepatica ADM28010_Ignisphaera_aggregans WP_ _Martelella_endophytica ADZ72558_Polymorphum_gilvum XP_ _Pyrenophora_teres AJY45479_Martelella_endophytica KIW25045_Cladophialophora_immunda WP_ _Ochrobactrum_sp XP_ _Bipolaris_maydis XP_ _Pestalotiopsis_fici KPQ17907_Rhodobacteraceae_bacterium WP_ _Rhizobium_sp WP_ _Thiothrix_flexilis WP_ _Hungatella_hathewayi KIW15468_Exophiala_spinifera XP_ _Sclerotinia_sclerotiorum XP_ _Leptosphaeria_maculans XP_ _Grosmannia_clavigera XP_ _Setosphaeria_turcica XP_ _Cladophialophora_yegresii KGK40729_Pichia_kudriavzevii WP_ _Celeribacter_indicus AJE45757_Celeribacter_indicus WP_ _Ochrobactrum_anthropi /Archaea VM1G_10361

34 0.3 WP_02663_Nocardia_brasiliensis KJR86022_Sporothrix_schenckii ERS929_Sporothrix_schenckii WP_ _Actinopolyspora_erythraea WP_ _Gordonia_effusa WP_ _Nocardia_brasiliensis WP_ _Mycobacterium_abscessus WP_ _Streptacidiphilus_anmyonensis WP_ _Mycobacterium_iranicum WP_ _Streptomyces_xylophagus WP_ _Nocardia_brasiliensis WP_ _Mycobacterium_gilvum WP_ _Mycobacterium_aromaticivorans XP_ _Togninia_minima WP_ _Mycobacterium_aurum WP_ _Nocardia_nova WP_ _Mycobacterium_vaccae WP_ _Sandarakinorhabdus_sp KIH915_Sporothrix_brasiliensis WP_ _Nocardia_vulneris KDE0_Mycobacterium_aromaticivorans WP_ _Streptomyces_monomycini VM1G_10685_Valsa_mali VM1G_10685

35 WP_ _Clostridiales_bacterium WP_ _Clostridiales_bacterium KON206_miscellaneous_Crenarchaeota WP_ _Moorella_thermoacetica WP_ _Anaerosalibacter_sp WP_ _Thermococcus_litoralis WP_ _Acetonema_longum WP_ _Caldivirga_maquilingensis WP_ _Pyrococcus_horikoshii CBK79287_Coprococcus_catus WP_ _Lysinibacillus_fusiformis WP_ _Blautia_producta WP_ _Lysinibacillus_sphaericus VM1G_109_Valsa_mali KON29082_miscellaneous_Crenarchaeota WP_ _bacterium_OL WP_ _Lysinibacillus_sphaericus WP_ _Pyramidobacter_piscolens WP_ _Blautia_producta WP_ _bacterium_UASB WP_00526_Blautia_hydrogenotrophica WP_ _Photobacterium_sanctipauli WP_ _Clostridiales_bacterium WP_ _Spirochaeta_bajacaliforniensis WP_ _Pyrococcus_sp WP_ _Anaerobaculum_hydrogeniformans Outgroup Outgroup /Archaea VM1G_109