Electronic Supplementary Material

Electronic Supplementary Material Table S1 Locality details, including geographical coordinates and altitudes, number of plants of the Cyanus triumfetti and C. montanus groups used in open pollination, isolation, emasculation and hybridization experiments Population code Cyanus montanus (L.) Hill MON 1 IT; Valle del Chisone valley, meadow close to Duc; 44 58 27 N, 06 53 27 E; 1,930 m MON 2 IT; Valle del Chisone valley, meadow close to Sestriere; 45 05 15 N, 06 26 09 E; 1,980 m MON 3 F; Massif du Mţ. Cenis Mts, Lac Du Mont Cenis lake; 45 14 38 N, 6 56 49 E; 2,108 m Locality details Open pollination Isolation experiments MON 6 F; Queyras Mts; meadow near Ceillac; 44 40 23 N, 6 46 10 E; 1,706 m Cyanus mollis (Waldst. & Kit.) J. Presl & C. Presl MOL 2 SK; Veľká Fatra Mts, Šturec saddle; 48 50 05 N, 18 59 43 E; 885 m MON 1/5 MON 1/2, MON 1/4, MON 1/5, MON 1/6 Emasculation experiments Intraspecies MON 1/2, MON 1/4, MON 1/5 Interspecies Heteroploid MON 1/4, MON 1/5 MON 2/2, MON 2/3 MON 2/1, MON 2/VI MON 2/VI MON 2/1, MON 2/2 MON 3A/3, MON 3B/19 MOL 2/1, MOL 2/5, MOL 2/II, MOL 2/III, MOL 2/IV, MOL 2/VI MOL 3/1, MOL 3/5, MOL 3/15 MON 3A/2, MON 3B/20 MOL 2/1, MOL 2/3,MOL 2/4, MOL 2/I, MOL 2/IV, MOL 2/VI, MOL 2/IX MON 3A/2 MON 3B/20, MON 3B/19 MON 3A/1, MON 3A/2, MON 3B/20, MON 3B/19 MON 6/1 MON 6/1, MON 6/7 MOL 2/I, MOL 2/IV MOL 2/III, MOL 2/IV MOL 2/1, MOL 2/3, MOL 2/5, MOL 2/III, MOL 2/IV, MOL 2/VI MOL 3 SK; Veporské vrchy Mts, Mt. Hradište near Brusno; 48 47 00 N, 19 20 31 E; 431 m MOL 3/1, MOL 3/15 MOL 3/5 MOL 3/1 MOL 4 SK; Spišsko Gemerský kras Karst, close to Vernár; MOL 4/1 MOL 4/5 MOL 4/2, MOL 48 54 38 N, 20 15 28 E; 870 m 4/5 MOL 5 SK, Belianske Tatry Mts, Dolina Siedmych prameňov MOL 5/1 MOL 5/1, MOL valley; 49 14 01 N, 20 16 39 E; 1,527 m 5B/6, MOL 5B/7 Cyanus triumfetti (All.) Dostál ex Á. Löve & D. Löve TRI 60 IT; Valle del Chisone valley, Forte Fenestrellum fort; TRI 60/12, TRI TRI 60/10, TRI 60/14 TRI 60/10, TRI 60/11, 45 01 58 N, 07 03 35 E; 1,303 m 60/13 TRI 60/13 TRI 61 IT; Valle del Chisone valley, meadow close to Ruá; TRI 61/1 TRI 61/1 TRI 61/2 45 01 22 N, 06 57 00 E; 1,499 m TRI 62 IT; Valle del Chisone Valley, Duc, meadow along the TRI 62/4 TRI 62/3, TRI 62/5 TRI 62/2, TRI 62/3, TRI TRI 62/6, TRI 62/7 road; 44 58 27 N, 06 53 27 E; 1,930 m 62/6 TRI 63 IT; Valle del Chisone valley, meadow close to TRI 63/2, TRI 63/4 Sestriere; 45 05 15 N, 06 26 09 E; 1,980 m TRI 64 IT; Valle del Chisone valley, close to Deveys; TRI 64/2, TRI 64/4 TRI 64/3 TRI 64/1, TRI 64/2, TRI 64/1, TRI 64/2, TRI TRI 64/7

45 05 01 N, 06 54 20 E; 1,079 m TRI 64/3, TRI 64/7 64/3, TRI 64/4 TRI 64/5 TRI 66 F; Liguria, Alpes Maritimes Mts., town of Tenda, TRI 66/2, TRI 66/5 TRI 66/1 TRI 66/2, TRI 66/4 TRI 66/4 along the road above the town; 44 04 16 N, 07 34 35 E; 1,194 m TRI 67 F; Alpes Maritimes Mts, road to Colle di Tende saddle; 44 06 03 N, 07 31 24 E; 2,013 m TRI 67/2 TRI 67/1 TRI 68 F; Liguria, Alpes Maritimes Mts., town of Tenda, along TRI 68/3 TRI 68/2 the unsurfaced road to Colle di Tende saddle, close to old buiding; 44 08 22 N, 07 33 00 E; 1,851 m TRI 69 IT; Valle del Chisone valley, settlement of TRI 69A/2, TRI TRI 69A/3 TRI 69A/4 TRI 69A/2, TRI 69A/4 Moncenisio; 45 11 20 N,06 59 20 E; 1,381 m 69A/4 TRI 106 CH; Wallis, meadow close to Blattern; 46 21 40 N, 07 58 49.4 E; 1,469 m TRI 106/1, TRI 106/2, TRI 106/3, TRI 106/5 TRI 107 CH; Wallis, above road to Ried bei Mörel; TRI 107/3 46 20 47.1 N, 08 01 10.9 E; 1,103 m Cyanus axillaris auct. TRI 4 SK; Považský Inovec Mts, S slope below the Tematín TRI 4/I, TRI 4/II, TRI 4/II TRI 4/V TRI 4/I, TRI 4/VI TRI 4/II Castle; 48 40 15 N, 17 57 45 E; 580 m TRI 4/VI TRI 7 SK; Podunajská nížina Lowlands, SW part of Mt. TRI 7/4 TRI 7/II Zobor near Nitra; 48 20 49 N, 18 05 40 E; 362 m TRI 8 SK; Podunajská nížina Lowlands, Mt. Šipka near TRI 8/I, TRI 8/II, TRI 8/I, TRI 8/II, TRI TRI 8/II, TRI 8/III TRI 8/I, TRI 8/II, TRI Plášťovce; 48 09 58 N, 18 59 47 E; 315 m TRI 8/III 8/III 8/III TRI 11 SK; slope close to Nižná Pokoradza, 48 24 51 N, TRI11/2, TRI 11/3, TRI11/2, TRI 11/5 TRI 11/5 18 01 48 E; 390 m TRI 11/5 TRI 15 HU; Mátra Mts., town of Gyöngyös, slopes in SW TRI 15/2, TRI 15/3 TRI 15/1, TRI 15/5, TRI 15/1, TRI 15/2, TRI 15/1, TRI 15/2, TRI part of Sár-Hegy Hill (499 m); 47 47 15 N, TRI 15/6 TRI 15/3 TRI 15/4, 15/3, TRI 15/6 19 57 35 E; 340 m TRI 15/5, TRI 15/6 TRI 16 TRI 17 TRI 23 TRI 34 TRI 41 SK; Považský Inovec Mts, Mt. Kamienka near Modrová; 48 38 37 N, 17 54 00 E; 245 m SK; Hornádska kotlina Basin, village of Žehra, SE part of Dreveník Hill (609.3 m); 48 58 48 N, 20 46 47 E; 558 m SK; Východoslovenská rovina Lowland, Mt. Veľký vrch near Brehov; 48 29 36 N, 21 48 35 E; 240 m SK; Belianske kopce Hills, near the top of Veľký vrch Hill; 47 49 45 N, 18 37 35 E; 240 m AU; Hainburger berge Mts, SW slope of Mt. Braunsberg; 48 09 15 N, 16 57 07 E; 291 m TRI 16/1, TRI 16/3, TRI 16/4 TRI 17/2, TRI 17/9 TRI 17/1, TRI 17/3, TRI 17/4 TRI 23/1, TRI 23/2, TRI 23/3, TRI 23/4, TRI 16/1, TRI 16/3 TRI 16/5 TRI 16/1, TRI 16/3, TRI 16/4, TRI 16/5 TRI 23/1, TRI 23/2, TRI 23/3, TRI 23/4, TRI 17/1 TRI 17/2, TRI 17/9 TRI 17/2, TRI 17/4 TRI 23/3 TRI 23/1, TRI 23/3, TRI 23/3, TRI 23/4 TRI 106/4, TRI 106/5 TRI 15/2 TRI 23/1, TRI 23/2, TRI 23/3, TRI 23/4 TRI 34/1 TRI 34/3 TRI 34/5 TRI 34/1, TRI 34/5 TRI 34/5 TRI 41/6 TRI 41/2, TRI 41/4, TRI 41/7 TRI 41/2, TRI 41/3, TRI 41/4, TRI 41/6 TRI 41/2, TRI 41/3, TRI 41/4, TRI 41/5, TRI 41/6 TRI 41/7

TRI 42 AU; Wienerwald Mts, Rauhenstein Castle near Baden; 48 00 46 N, 16 12 27 E; 324 m TRI 43 AU; Drosendorf Stadt, view point 1.5 km N of the town; 48 52 27 N, 15 37 52 E; 434 m TRI 45 CZ; Moravská vrchovina Mts, rocks NE of Moravský Krumlov; 49 03 39 N, 16 19 40 E; 283 m TRI 46 CZ; Moravská vrchovina Mts, rocks near Ivančice; 49 05 20 N, 16 20 25 E; 258 m TRI 47 CZ; Pavlovské vrchy Mts, Mt. Děvín near Horní Věstonice; 48 51 53 N, 16 38 37 E; 424 m TRI 48 CZ; Pavlovské vrchy Mts, Svatý kopeček hill near Mikulov; 48 48 24 N, 16 38 44 E; 356 m TRI 49 CZ; Biele Karpaty Mts, Žerotín hill near Radějov; 48 51 47 N, 17 19 38 E; 321 m TRI 70 SK; Záhorská nížina Lowlands, loc. Široká near Malacky, 48 24 44 N, 17 04 14 E; 193 m TRI 77 HU; Mecsek Mts, road to television tower (Misina) near Pécs, 46 05 59 N, 18 13 03 E; 522 m TRI 84 HU; Budai-hegység Mts, SW slope of Mt. Kis-hárshegy; 47 31 46 N, 18 57 55 E; 357 m TRI 86 CZ; Český kras karst, slope close to České Srbsko; 49 55 52 N, 14 08 25 E, 236 m TRI 87 AU; Wiener Wald Mts, Mt. Leopoldsberg near Klosterneuburg; 48 16 41 N, 16 20 38 E; 397 m TRI 93 CZ; České Středohoří Mts, E margin of Hlinná; 50 34 23 N, 14 06 44 E; 464 m TRI 95 SK, Biele Karpaty Mts, Mt. Veterník near Skalica; 48 48 59 N, 17 13 49 E; 312 m Cyanus strictus (Waldst. & Kit.) Soják TRI 22 HU; Zempléni-hegység Mts, hill with a cross above Tállya; 48 16 09 N, 21 11 35 E; 322 m TRI 24 TRI 35 TRI 37 SK; Vihorlatské vrch Mts., SW slope below Viniansky hrad Castle, 48 49 10 N, 21 56 53 E; 295 m HU; Bükk Mts, SE part of Mt. Bel-kö near Bélapátfalva; 48 02 30 N, 20 22 10 E; 630 m HU; Zempléni-hegység Mts, hill with a cross above Erdöbénye; 48 15 40 N, 21 21 40 E; 227 m TRI 48/1, TRI 48/2, TRI 48/6 TTI 42/7 TRI 43/2 TRI 43/7 TRI 43/2, TRI 43/5, TRI 43/7 TRI 45/4 TRI 45/1, TRI 45/4, TRI 45/5 TRI 46/6 TRI 46/4, TRI 46/6 TRI 46/3, TRI 46/4, TRI TRI 46/3 46/5, TRI 46/6, TRI 46/7 TRI 47/4, TRI 47/6 TRI 47/4, TRI 47/6, TRI 47/2, TRI 47/7 TRI 47/8 TRI 48/4 TRI 48/1, TRI 48/5 TRI 48/8 TRI 48/6 TRI 49/2 TRI 49/1, TRI 49/6 TRI 49/6 TRI 49/1, TRI 49/6 TRI 49/1, TRI 49/5, TRI 49/6 TRI 70/1 TRI 70/1, TRI 70/2, TRI 70/1, TRI 70/6 TRI 70/6 TRI 77/1, TRI 77/4 TRI 22/1, TRI 22/3, TRI 22/4 TRI 24/1, TRI 24/2, TRI 24/3, TRI 24/4 TRI 22/1, TRI 22/2, TRI 22/3 TRI 22/4, TRI 22/5 TRI 24/1, TRI 24/2, TRI 24/5 TRI 37/2 TRI 37/2, TRI 37/3, TRI 37/4, TRI 37/5 TRI 24/1, TRI 24/2 TRI 84/4 TRI 86/2 TRI 86/1 TRI 95/3, TRI 95/4 TRI 22/1 TRI 24/2, TRI 24/3, TRI 24/5 TRI 49/3 TRI 87/1 TRI 87/3 TRI 93/1, TRI 93/5 TRI 95/2, TRI 95/3, TRI 95/5 TRI 22/1, TRI 22/3, TRI 22*/3, TRI 22*/4 TRI 24/1, TRI 24/2, TRI 24/4, TRI 24/5 TRI 35/3 TRI 35/1, TRI 35/2 TRI 37/4 TRI 37/1

TRI 38 HU; Zempléni-hegység Mts, in vineyards by Tolcsva; TRI 38/4, TRI 38/7 TRI 38/3, TRI 38/6, TRI 38/5, TRI 38/6 TRI 38/2, TRI 38/7 TRI 38/2 48 16 13 N, 21 23 18 E; 159 m TRI 38/7 TRI 39 SK; Zemplínske vrchy Mts, E slope of Mt. Piliš near TRI 39/4, TRI 39/19 TRI 39/1, TRI 39/4 TRI 39/2, TRI 39/16, TRI 39/1, TRI 39/2, TRI TRI 39/16, TRI Veľká Bára; 48 25 52 N, 21 42 45 E; 224 m TRI 39/16, TRI 39/17 TRI 39/17 TRI 39/19 39/16, TRI 39/19 39/16 TRI 40 SK; Zemplínske vrchy Mts, Mt. Šimonov vrch near Malá Tŕňa; 48 26 28 N, 21 41 23 E; 173 m TRI 40/2, TRI 40/4, TRI 40/5 TRI 40/1, TRI 40/2, TRI 40/4, TRI 40/5 TRI 40/5 TRI 40 Cyanus dominii (Dostál) Holub subsp. dominii TRI 25 SK; Volovské vrchy Mts, Mt. Humenec near Veľká TRI 25/3, TRI 25/4, TRI 25/1, TRI 25/2, TRI 25/4, TRI 25/5 Lodina; 48 51 34 N, 21 09 33 E; 280 m TRI 25/8 TRI 25/3, TRI 25/8 TRI 54 SK; Slovenský raj Mts, Čingov; 48 56 35 N, 20 28 58 E; 337 m TRI 54/7 TRI 55 SK; Branisko Mts, Lačnov canyon near Lipovec; 49 03 20 N, 20 56 12 E; 585 m TRI 55/5, TRI 55/10 TRI 55/5, TRI 55/6 TRI 55/10 TRI 55/6 TRI 55/5, TRI 55/7 TRI 55/1, TRI 55/2, TRI 55/6, TRI 55/8, TRI 55/11 TRI 57 SK; Volovské vrchy Mts, rocks near Malá Lodina; TRI 57/9 TRI 57/2, TRI 57/6, TRI 57/10 TRI 57/11 48 51 51 N, 21 05 54 E; 950 m TRI 57/7, TRI 57/9 TRI 110 SK; Branisko Mts, pine forest on the W slope of Mt. Rajtopíky; 49 00 00 N, 20 51 37 E; 950 m TRI 110/2 Cyanus dominii subsp. slovenicus (Dostál) Olšavská TRI 3 SK; Podtatranská kotlina Basin, Mt. Mních near TRI 3/III, TRI 3/IV, TRI 3/II, TRI 3/IV TRI 3/II TRI 3/IIITRI 3/V, TRI 3/II TRI 3/II Ružomberok; 49 05 15 N, 19 19 55 E; 500 m TRI 3/V TRI 3/VI TRI 6 SK; Turčianska kotlina Basin, rocks by the road to Šútovo; 49 08 50 N, 19 05 30 E; 500 m TRI 6/I, TRI 6/II, TRI 6/III, TRI 6/IV TRI 6/I, TRI 6/II TRI 6/II TRI 6/IV TRI 6/IV TRI 13 SK; Veľká Fatra Mts, central part of Gaderská dolina TRI 13/4 TRI 13/1, TRI 13/3, TRI valley; 48 56 40 N, 19 00 35 E ; 580 m 13/4 TRI 14 SK; Horehronské podolie Basin, Mt. Horné lazy near TRI 14/2 TRI 14/2, TRI 14/3, TRI 14/1, TRI 14/2, TRI Valaská; 48 49 40 N, 19 36 00 E; 620 m TRI 14/4, TRI 14/5 14/3, TRI 14/4 TRI 18 SK; Spišsko Gemerský kras Karst, ravine by Vernár; TRI 18/3 TRI 18/2, TRI 18/11 48 56 00 N, 20 17 15 E; 760 m TRI 19 SK; Chočské vrchy Mts, Prosiecká dolina valley near TRI 19/1, TRI 19/2 TRI 19/2 TRI 19/4 TRI 19/1, TRI 19/2, TRI 19/1, TRI 19/2, TRI Prosiek; 49 09 00 N, 19 29 55 E; 640 m TRI 19/4 19/4 TRI 26 SK; Spišský Štiavnik slope below railway station; TRI 26/2 TRI 26/3 TRI 26/1, TRI 26/2 49 00 19 N, 20 22 47 E; 570 m TRI 27 SK; Nízke Tatry Mts, Mt. Vachtárová near Kráľová TRI 27/2, TRI 27/3, TRI 27/2, TRI 27/4, TRI 27/1, TRI 27/2, TRI 27/3, Lehota; 49 01 25 N, 19 48 05 E; 810 m TRI 32 SK; Chočské vrchy Mts, Sokol Hill; 49 08 16 N, TRI 32/1, TRI 32/6 19 18 38 E; 1,100 m TRI 53 SK; Súľovské vrchy Mts, cottage Súľovčanka near Súľov; 49 10 05 N, 18 34 35 E; 410 m TRI 53/2

Cyanus dominii subsp. sokolensis (Pawł.) Olšavská TRI 5 SK; Slovenský kras Karst, hill above Turňa nad Bodvou; 48 36 43 N, 20 52 20 E; 349 m TRI 21 SK; Slovenský kras Karst, E slope above Slavec; 48 35 59 N, 20 27 59 E; 312 m TRI 33 SK; Západné Tatry Mts, Mt. Mních near Bobrovec; 49 10 40 N, 19 38 45 E; 1,380 m TRI 44 SK; Západné Tatry Mts, Mt. Sokol near Bobrovec; 49 10 29 N, 19 38 17 E; 1,325 m TRI 50 SK; Slovenský kras Karst, Zádielská dolina valley near Zádiel; 48 37 37 N, 20 50 01 E; 567 m TRI 51 SK; Slovenský kras Karst, Mt. Zajačia strana hill; 48 35 15 N, 20 43 40 E; 250 m intermediate morphotype between Cyanus axillaris and Cyanus dominii TRI 9 SK; Slovenský kras Karst, limestone rocks above Domica Cave; 48 28 40 N, 20 28 15 E; 359 m TRI 10 SK; Slovenský kras Karst, SW slope below Krásna Hôrka Castle; 48 39 31 N, 20 35 55 E; 427 m TRI 20 SK; Slovenský kras Karst, meadow W of Gemerská Hôrka; 48 32 25 N, 20 21 01 E; 282 m TRI 52 SK; Slovenský kras Karst, Mt. Veľký Paklán; 48 35 18 N, 20 43 30 E; 253 m TRI 31 SK; Pieniny Mts, SW part of Mt. Holica near Lesnica; 49 24 35 N, 20 26 20 E; 540 m Cyanus adscendens (Bartl.) Soják TRI 71 SLO; Nanos Mts., pine forest above Podnanos; 45 47 48 N, 14 00 39 E; 803 m TRI 72 SLO; Nanos Mts., summit Plesa; 45 46 21 N, 14 03 06 E; 1,230 m Cyanus pinnatifidus (Schur) Holub TRI 73 RU; Poiana Braşov Mts., Tâmpa hill; 45 38 03 N, 25 35 32 E, 955 m TRI 139 RU; Poiana Braşov Mts., Ruia peak; 45 34 28 N, 25 33 46 E; 1,655 m TRI 5/III TRI 5/7, TRI 5/III TRI 5/8 TRI 5/1, TRI 5/2, TRI 5/3, TRI 5/6 TRI 21/3 TRI 21/2, TRI 21/3 TRI 21/1, TRI 21/3 TRI 21/1, TRI 21/2, TRI 21/5 TRI 9A/2, TRI 9A/3, TRI 9A/7, TRI 9A/8 TRI 10/1, TRI 10/2, TRI 10/3, TRI 10/4 TRI 33/1, TRI 33/6 TRI 33/4 TRI 44/1 TRI 44/1, TRI 44/3, TRI 44/5, TRI 44/8 TRI 50/1, TRI 50/2 TRI 50/2, TRI 50/3, TRI 50/6 TRI 51/1 TRI 51/1, TRI 51/3, TRI 51/4, TRI 51/5 TRI 9A/2, TRI 9A/7, TRI 9A/9 TRI 10/1, TRI 10/2, TRI 10/3 TRI 10/2, TRI 10/3 TRI 9A/2, TRI 9A/3, TRI 9A/8, TRI 9/I, TRI 9/II TRI 10/1, TRI 10/3, TRI 10/4, TRI 10/13, TRI 10/14, TRI 140/15 TRI 20/3 TRI 20/1, TRI 20/2, TRI 20/3, TRI 20*/4 TRI 52/5 TRI 52/5 TRI 52/1, TRI 52/2 TRI71/2 TRI71/1, TRI71/2, TRI71/3, TRI71/5, TRI71/6 TRI 31/1, TRI 31/3, TRI 31/4 TRI 71/1, TRI 71/2, TRI 71/4, TRI 71/5, TRI 71/6 TRI 51/2, TRI 51/3, TRI 51/4 TRI72/1 TRI72/2 TRI 72/1, TRI 72/3 TRI 72/1, TRI 72/3 TRI 139/1, TRI 139/3, TRI 139/5 TRI 139/1, TRI 139/2, TRI 139/3, TRI 139/5 TRI 73/4, TRI 73/5, TRI 73/6, TRI 73/7 TRI 139/5