Current Weed Control strategies in sorghum I In Pre-planting/pre-emergence chemichal fallow use of seed safeners residual herbicides efficacy uncertain high dependency on rains
Current Weed Control strategies in sorghum II In Post-emergency parcial control of broad leaf weeds non existence of graminicides Diagnostic Almost nothing to do with weeds!!!
Usual failures in weed control in Sorghum I
Usual failures in weed control in Sorghum II
Usual failures in weed control in Sorghum III
How to Maximize Advanta s Competitive Genetic Gain Thailand October 2007 Argentina Sorghum R&D Team Argentina Sorghum Breeding Program New technologies for a new sorghum crop Non transgenic herbicides resistance Mutagenic Program started
Grain 2007 2009 2010 2011 2012 Mutagenesis Program started on elite lines (BG) First selections M3 First introgression crosses Active ingredients & dosis. Genetic characterization & heritability Gene secquencing & Gene mapping in Mol Lab. Grain & biomass Yield drag field trials
M3 generation resistant mutation Summer 2008/09, Venado Tuerto, Argentina - one of thousands rows
Mol Lab: mutation sequencing /align 1 SbAHAS-F1 Start Codon 100 ---------------------------------------------------------------------------------------------------- ADV-IMI-R (1) 80237 (1) GTGCCCCCGCCCCAAACCCTCGCGCCGCCTCCGAGACAGCCGCCGCAACCATGGCCACCACCGCCGCCGCCGCTGCCGCCGCGCTAGCCGGCGCCACTAC 101 200 ---------------------------------------------------------------------------------------------------- ADV-IMI-R (1) 80237 (101) CGCTGCGCCCAAGGCGAGGCGCCGGGCGCACCTCCTGGCCGCACGGCGCGCCCTCGCCGCGCCCATCAGGTGCTCAGCGGCGCCACCCGCCACGCTGACG 201 SbAHAS-F1-2 300 ADV-IMI-R (1) -------------------------------------------------------------------------TCGAGGCTCTTGAGCGCTGCGGCGTCC (201) GTGACGGCTCCCCCGGCCACCCCGCTCCGGCCGTGGGGCCCCACCGATCCCCGCAAGGGCGCCGACATCCTCGTCGAGGCTCTTGAGCGCTGCGGCGTCC 80237 301 G A 400 ADV-IMI-R (28) GCGACGTCTTCGCCTACCCCGGCGGCACGTCCATGGAGATCCACCAGGCACTCACCCGTTCCCCCGTCATCGCCAACCACCTCTTCCGCCACGAGCAAGG (301) GCGACGTCTTCGCCTACCCCGGCGGCGCGTCCATGGAGATCCACCAGGCACTCACCCGTTCCCCCGTCATCGCCAACCACCTCTTCCGCCACGAGCAAGG 80237 401 500 ADV-IMI-R (128) GGAGGCCTTCGCCGCCTCTGGCTTCGCGCGCTCCTCGGGCCGCGTCGGCGTCTGCGTCGCCACCTCCGGCCCCGGCGCCACCAACCTAGTCTCCGCGCTC (401) GGAGGCCTTCGCCGCCTCTGGCTTCGCGCGCTCCTCGGGCCGCGTCGGCGTCTGCGTCGCCACCTCCGGCCCCGGCGCCACCAACCTAGTCTCCGCGCTC 80237 501 SbAHAS-F2 SbAHAS-R1 600 ADV-IMI-R (228) GCCGACGCGCTGCTCGACTCCGTCCCCATGGTCGCCATCACGGGACAGGTTCCGCGGCGCATGATTGGCACCGACGCCTTCCAGGAGACGCCCATCGTCG (501) GCCGACGCGCTGCTCGACTCCGTCCCCATGGTCGCCATCACGGGACAGGTTCCGCGGCGCATGATTGGCACCGACGCCTTCCAGGAGACGCCCATCGTCG 80237 601 700 ADV-IMI-R (328) AGGTCACCCGCTCCATCACCAAACATAACTACCTGGTCCTCGACGTCGACGACATCCCCCGCGTCGTGCAGGAGGCTTTCTTCCTCGCCTCCTCCGGTCG (601) AGGTCACCCGCTCCATCACCAAACATAACTACCTGGTCCTCGACGTCGACGACATCCCCCGCGTCGTGCAGGAGGCTTTCTTCCTCGCCTCCTCCGGTCG 80237 701 800 ADV-IMI-R (428) CCCGGGACCGGTGCTTGTCGACATCCCCAAGGACATCCAGCAGCAGATGGCCGTGCCGGTCTGGGACACGCCCATGAGTCTGCCTGGGTACATTGCGCGC (701) CCCGGGACCGGTGCTTGTCGACATCCCCAAGGACATCCAGCAGCAGATGGCCGTGCCGGTCTGGGACACGCCCATGAGTCTGCCTGGGTACATTGCGCGC 80237 801 900 ADV-IMI-R (528) CTTCCCAAGCCTCCTGCGACTGAATTGCTTGAGCAGGTGCTGCGTCTTGTTGGTGAATCAAGGCGCCCTGTTCTTTATGTTGGTGGTGGCTGCGCAGCAT (801) CTTCCCAAGCCTCCTGCGACTGAATTGCTTGAGCAGGTGCTGCGTCTTGTTGGTGAATCAAGGCGCCCTGTTCTTTATGTTGGTGGTGGCTGCGCAGCAT 80237 901 1000 ADV-IMI-R (628) CTGGCGAGGAGTTGCGCCGCTTTGTGGAGATGACTGGAATCCCAGTCACAACTACTCTTATGGGCCTTGGCAATTTCCCTGGCGACGACCCACTGTCTCT (901) CTGGCGAGGAGTTGCGCCGCTTTGTGGAGATGACTGGAATCCCAGTCACAACTACTCTTATGGGCCTTGGCAATTTCCCTGGCGACGACCCACTGTCTCT 80237 1001 SbAHAS-F3 SbAHAS-R2 1100 ADV-IMI-R (728) GCGCATGCTTGGTATGCATGGCACGGTGTATGCAAATTATGCAGTGGATAAGGCGGATCTGTTGCTTGCATTTGGTGTGCGGTTTGATGATCGTGTGACA 80237 (1001) GCGCATGCTTGGTATGCATGGCACGGTGTATGCAAATTATGCAGTGGATAAGGCGGATCTGTTGCTTGCATTTGGTGTGCGGTTTGATGATCGTGTGACA 1101 1200 ADV-IMI-R (828) GGGAAGATTGAGGCTTTTGCAAGCAGGGCTAAGATTGTGCACATTGATATTGATCCCGCTGAGATTGGCAAGAACAAGCAGCCACATGTGTCCATCTGTG 80237 (1101) GGGAAGATTGAGGCTTTTGCAAGCAGGGCTAAGATTGTGCACATTGATATTGATCCCGCTGAGATTGGCAAGAACAAGCAGCCACATGTGTCCATCTGTG 1201 1300 ADV-IMI-R (928) CAGACGTTAAGCTTGCTTTGCAGGGCATGAATGCTCTTCTGGAAGGAAGCACATCAAAGAAGAGCTTTGACTTTGGCTCATGGCAAGCTGAGTTGGATCA 80237 (1201) CAGACGTTAAGCTTGCTTTGCAGGGCATGAATGCTCTTCTGGAAGGAAGCACATCAAAGAAGAGCTTTGACTTTGGCTCATGGCAAGCTGAGTTGGATCA 1301 1400 ADV-IMI-R (1028) GCAGAAGAGAGAGTTCCCCCTTGGGTATAAAACTTTTGATGACGAGATCCAGCCACAATATGCTATTCAGGTTCTTGATGAGCTGACAAAAGGGGAGGCC 80237 (1301) GCAGAAGAGAGAGTTCCCCCTTGGGTATAAAACTTTTGATGACGAGATCCAGCCACAATATGCTATTCAGGTTCTTGATGAGCTGACAAAAGGGGAGGCC 1401 SbAHAS-F4 1500 ADV-IMI-R (1128) ATCATTGCCACAGGTGTTGGGCAGCACCAGATGTGGGCGGCACAGTACTACACTTACAAGCGGCCAAGGCAGTGGTTGTCTTCAGCTGGTCTTGGGGCTA 80237 (1401) ATCATTGCCACAGGTGTTGGGCAGCACCAGATGTGGGCGGCACAGTACTACACTTACAAGCGGCCAAGGCAGTGGTTGTCTTCAGCTGGTCTTGGGGCTA 1501 SbAHAS-R3 1600 ADV-IMI-R (1228) TGGGATTTGGTTTGCCGGCTGCTGCTGGCGCTGCTGTGGCCAACCCAGGTATCACTGTTGTTGACATCGACGGAGATGGTAGCTTCCTCATGAACATTCA 80237 (1501) TGGGATTTGGTTTGCCGGCTGCTGCTGGCGCTGCTGTGGCCAACCCAGGTATCACTGTTGTTGACATCGACGGAGATGGTAGCTTCCTCATGAACATTCA 1601 SbAHAS-F5 1700 ADV-IMI-R (1328) GGAGCTAGCTATGATCCGAATTGAGAACCTCCCAGTGAAGGTCTTTGTGCTAAACAACCAGCACCTGGGGATGGTGGTGCAGTGGGAGGACAGGTTCTAT 80237 (1601) GGAGCTAGCTATGATCCGAATTGAGAACCTCCCAGTGAAGGTCTTTGTGCTAAACAACCAGCACCTGGGGATGGTGGTGCAGTGGGAGGACAGGTTCTAT 1701 1800 ADV-IMI-R (1428) AAGGCCAATAGAGCACACACATACTTGGGAAACCCAGAGAATGAAAGTGAGATATATCCAGATTTCGTGACAATTGCCAAAGGGTTCAACATTCCAGCAG 80237 (1701) AAGGCCAATAGAGCACACACATACTTGGGAAACCCAGAGAATGAAAGTGAGATATATCCAGATTTCGTGACAATTGCCAAAGGGTTCAACATTCCAGCAG 1801 1900 ADV-IMI-R (1528) TCCGTGTGACAAAGAAGAGCGAAGTCCATGCAGCAATCAAGAAGATGCTTGAGACTCCAGGGCCATACCTCTTGGATATAATCGTCCCGCACCAGGAGCA 80237 (1801) TCCGTGTGACAAAGAAGAGCGAAGTCCATGCAGCAATCAAGAAGATGCTTGAGACTCCAGGGCCATACCTCTTGGATATAATCGTCCCGCACCAGGAGCA 1901 SbAHAS-R4 Stop Codon 2000 ADV-IMI-R (1628) TGTGTTGCCTATGATCCCTAGTGGTGGGGCTTTCAAGGATATGATCCTGGATGGTGATGGCAGGACTGTGTATTGATCTAAATTTCAGCATGCACATCTC 80237 (1901) TGTGTTGCCTATGATCCCTAGTGGTGGGGCTTTCAAGGATATGATCCTGGATGGTGATGGCAGGACTGTGTATTGATCTAAATTTCAGCATGCACATCTC 2001 2100 ADV-IMI-R (1728) CCTGCCTTTCTTTGACATGCATATGAGCTGGTACAAGGGTGATGTGTTATTTATGTGATGTTCTCCTGTGTTCTATCTTTTTGTAAGCCGTCAGCTATCT 80237 (2001) CCTGCCTTTCTTTGACATGCATATGAGCTGGTACAAGGGTGATGTGTTATTTATGTGATGTTCTCCTGTGTTCTATCTTTTTGTAAGCCGTCAGCTATCT 2101 2200 ADV-IMI-R (1828) ATAGTGTGCTTGTTTGATGTACTCTGTTATGGTAATCTTAAGTAGTTTCCTACCTTGTAGTGGTGTAGTCTGTTGTTTCGTGCTGGCATATCTGTCATCA 80237 (2101) ATAGTGTGCTTGTTTGATGTACTCTGTTATGGTAATCTTAAGTAGTTTCCTACCTTGTAGTGGTGTAGTCTGTTGTTTCGTGCTGGCATATCTGTCATCA 2201 SbAHAS-R5 2274 ADV-IMI-R (1928) GAGGTCATGTAAGTGCCTTTTGCTACAGATAAATAAGGAAATAAGCATTGCTATGCAGTGGTTCTGTACGCCTC 80237 (2201) GAGGTCATGTAAGTGCCTTTTGCTACAGATAAATAAGGAAATAAGCATTGCTATGCAGTGGTTCTGTACGCCTC
Mol Lab: protein comparisons 1 100 ATH (1) MAAATTTTTTSSSISFSTKPSPSSSKSPLPISRFSLPFSLNPNKSSSSSRRRGIKSSSPSSISAVLNTTTNVTTTPSPTKPTKPETFISRFAPDQPRKGA 80237 (1) --MATTAAAAAAALAGATTAAP-------------------------KARRRAHLLAARRALAAPIRCSAAPPATLTVTAP--PATPLRPWGPTDPRKGA ADV-IMI-R (1) --MATTAAAAAAALAGATTAAP-------------------------KARRRAHLLAARRALAAPIRCSAAPPATLTVTAP--PATPLRPWGPTDPRKGA 101 cd122 (Ala122Tyr) 200 ATH (101) DILVEALERQGVETVFAYPGGASMEIHQALTRSSSIRNVLPRHEQGGVFAAEGYARSSGKPGICIATSGPGATNLVSGLADALLDSVPLVAITGQVPRRM 80237 (72) DILVEALERCGVRDVFAYPGGASMEIHQALTRSPVIANHLFRHEQGEAFAASGFARSSGRVGVCVATSGPGATNLVSALADALLDSVPMVAITGQVPRRM ADV-IMI-R (72) DILVEALERCGVRDVFAYPGGTSMEIHQALTRSPVIANHLFRHEQGEAFAASGFARSSGRVGVCVATSGPGATNLVSALADALLDSVPMVAITGQVPRRM 201 300 ATH (201) IGTDAFQETPIVEVTRSITKHNYLVMDVEDIPRIIEEAFFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMRLPGYMSRMPKPPEDSHLEQIVRLISESK 80237 (172) IGTDAFQETPIVEVTRSITKHNYLVLDVDDIPRVVQEAFFLASSGRPGPVLVDIPKDIQQQMAVPVWDTPMSLPGYIARLPKPPATELLEQVLRLVGESR ADV-IMI-R (172) IGTDAFQETPIVEVTRSITKHNYLVLDVDDIPRVVQEAFFLASSGRPGPVLVDIPKDIQQQMAVPVWDTPMSLPGYIARLPKPPATELLEQVLRLVGESR 301 400 ATH (301) KPVLYVGGGCLNSSDELGRFVELTGIPVASTLMGLGSYPCDDELSLHMLGMHGTVYANYAVEHSDLLLAFGVRFDDRVTGKLEAFASRAKIVHIDIDSAE 80237 (272) RPVLYVGGGCAASGEELRRFVEMTGIPVTTTLMGLGNFPGDDPLSLRMLGMHGTVYANYAVDKADLLLAFGVRFDDRVTGKIEAFASRAKIVHIDIDPAE ADV-IMI-R (272) RPVLYVGGGCAASGEELRRFVEMTGIPVTTTLMGLGNFPGDDPLSLRMLGMHGTVYANYAVDKADLLLAFGVRFDDRVTGKIEAFASRAKIVHIDIDPAE 401 500 ATH (401) IGKNKTPHVSVCGDVKLALQGMNKVLENRAEELKLDFGVWRNELNVQKQKFPLSFKTFGEAIPPQYAIKVLDELTDGKAIISTGVGQHQMWAAQFYNYKK 80237 (372) IGKNKQPHVSICADVKLALQGMNALLEGSTSKKSFDFGSWQAELDQQKREFPLGYKTFDDEIQPQYAIQVLDELTKGEAIIATGVGQHQMWAAQYYTYKR ADV-IMI-R (372) IGKNKQPHVSICADVKLALQGMNALLEGSTSKKSFDFGSWQAELDQQKREFPLGYKTFDDEIQPQYAIQVLDELTKGEAIIATGVGQHQMWAAQYYTYKR 501 cd562 cd574 600 ATH (501) PRQWLSSGGLGAMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQELATIRVENLPVKVLLLNNQHLGMVMQWEDRFYKANRAHTFLGDPAQEDEIFPN 80237 (472) PRQWLSSAGLGAMGFGLPAAAGAAVANPGITVVDIDGDGSFLMNIQELAMIRIENLPVKVFVLNNQHLGMVVQWEDRFYKANRAHTYLGNPENESEIYPD ADV-IMI-R (472) PRQWLSSAGLGAMGFGLPAAAGAAVANPGITVVDIDGDGSFLMNIQELAMIRIENLPVKVFVLNNQHLGMVVQWEDRFYKANRAHTYLGNPENESEIYPD 601 670 ATH (601) MLLFAAACGIPAARVTKKADLREAIQTMLDTPGPYLLDVICPHQEHVLPMIPSGGTFNDVITEGDGRIKY 80237 (572) FVTIAKGFNIPAVRVTKKSEVHAAIKKMLETPGPYLLDIIVPHQEHVLPMIPSGGAFKDMILDGDGRTVY ADV-IMI-R (572) FVTIAKGFNIPAVRVTKKSEVHAAIKKMLETPGPYLLDIIVPHQEHVLPMIPSGGAFKDMILDGDGRTVY
Field trials I: weed control effectiveness
Field trials II: imi herbicide tolerance
Field trials III: grain/biomass yield penalties
Grain yield [tn/ha] Yield drag I: isohybrids imi-non imi grain yield no sprayed 16 15 14 13 12 11 10 R1 R2 R3 S
Grain yield [tn/ha] Yield drag II: isohybrids imi grain yield sprayed no sprayed 17 16 15 14 13 12 11 10 R S R R R R R R R R R R R S R R R R R R R R R R CONTROL H1 H1 H2 H2 H3 H3 H4 H4 H5 H5 1x 2x 1x 2x 1x 2x 1x 2x 1x 2x
Dry matter +_ SD [tn/ha] Yield drag I: isohybrids biomass dry matter yield 70 60 50 40 30 20 10 R1 R2 R3 S R1 R2 R3 S R1 R2 R3 S R1 R2 R3 S R1 R2 R3 S R1 R2 R3 S R1 R2 R3 S R1 R2 R3 S R1 R2 R3 S R1 R2 R3 S R1 R2 R3 S CONTROL H1 H1 H2 H2 H3 H3 H4 H4 H5 H5 1x 2x 1x 2x 1x 2x 1x 2x 1x 2x
Tech to allow expressing yield potential
Tech to allow expressing yield potential
Igrowth Technology main features: Non transgenic Specific mutation: no tolerance to sulfonylurea and so controlled by sulfonylureas Recessive trait: homocigocity needed Mutacion has no impact in yield neither grain nor dry matter with or without herbicide application Post emergence alternative: unique Safe technology very well known by farmers
Part of the team responsible for success
THANK YOU vicente.trucillo@advantaseeds.com