Multiple Cloning Site. Venus Venus 1-172

Átírás

1 nzymes : 46 of 242 enzymes (Filtered) ettings: Linear, Certain Sites Only, Standard Genetic Code EcoICRI SacI Page 1 HindIII GAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTGATCTACCATGGACTACAAAGACGATGACGACAAGC â â â â â â â 79 CTCCAGATATATTCGTCTCGAGCAAATCACTTGGCAGTCTTAACTAGATGGTACCTGATGTTTCTGCTACTGCTGTTCG Ampicillin Resistant Plasmid FLAG-TAG E V Y I S R A R L V N R Q N. S T M D Y K D D D D K ßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß EagI NotI EcoRI ClaI BglII EcoRV Acc65I KpnI SalI AccI XbaI TTGCGGCCGCGAATTCATCGATAGATCTGATATCGGTACCAGTCGACTCTAGAAGATCCATCGCCACCATGGTGAGCAA â â â â â â â â 158 AACGCCGGCGCTTAAGTAGCTATCTAGACTATAGCCATGGTCAGCTGAGATCTTCTAGGTAGCGGTGGTACCACTCGTT Multiple Cloning Site Venus L A A A N S S I D L I S V P V D S R R S I A T M V S K GGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTG â â â â â â â â 237 CCCGCTCCTCGACAAGTGGCCCCACCACGGGTAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCAC G E E L F T G V V P I L V E L D G D V N G H K F S V TCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGCTGATCTGCACCACCGGCAAGCTGCCCGTGC â â â â â â â â 316 AGGCCGCTCCCGCTCCCGCTACGGTGGATGCCGTTCGACTGGGACTTCGACTAGACGTGGTGGCCGTTCGACGGGCACG S G E G E G D A T Y G K L T L K L I C T T G K L P V

2 Page 2 PstI CCTGGCCCACCCTCGTGACCACCCTGGGCTACGGCCTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGA â â â â â â â â 395 GGACCGGGTGGGAGCACTGGTGGGACCCGATGCCGGACGTCACGAAGCGGGCGATGGGGCTGGTGTACTTCGTCGTGCT P W P T L V T T L G Y G L Q C F A R Y P D H M K Q H D CTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACC â â â â â â â â 474 GAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGCGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGG F F K S A M P E G Y V Q E R T I F F K D D G N Y K T CGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCA â â â â â â â â 553 GCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCTGCCGT R A E V K F E G D T L V N R I E L K G I D F K E D G éßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßß ACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCAT â â â â â â â â 632 TGTAGGACCCCGTGTTCGACCTCATGTTGATGTTGTCGGTGTTGCAGATATAGTGGCGGCTGTTCGTCTTCTTGCCGTA N I L G H K L E Y N Y N S H N V Y I T A D K Q K N G I ßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßß

3 Page 3 BamHI XmaI SmaI CAAGGCCAACTTCAAGATCCGCCACAACATCGAGTAGGGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCT â â â â â â â â 711 GTTCCGGTTGAAGTTCTAGGCGGTGTTGTAGCTCATCCCTAGGGCCCACCGTAGGGACACTGGGGAGGGGTCACGGAGA K A N F K I R H N I E. G S R V A S L. P L P S A S ßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ß CCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAG â â â â â â â â 790 GGACCGGGACCTTCAACGGTGAGGTCACGGGTGGTCGGAACAGGATTATTTTAATTCAACGTAGTAAAACAGACTGATC P G P G S C H S S A H Q P C P N K I K L H H F V. L ßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß BbsI EcoO109I GTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCT â â â â â â â 869 CACAGGAAGATATTATAATACCCCACCTCCCCCCACCATACCTCGTTCCCCGTTCAACCCTTCTGTTGGACATCCCGGA G V L L. Y Y G V E G G G M E Q G A S W E D N L. G L ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß GCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGC â â â â â â â â 948 CGCCCCAGATAACCCTTGGTTCGACCTCACGTCACCGTGTTAGAACCGAGTGACGTTAGAGGCGGAGGACCCAAGTTCG R G L L G T K L E C S G T I L A H C N L R L L G S S BbvCI Bpu10I BlpI BsmBI GATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGG â â â â â â â â 1027 CTAAGAGGACGGAGTCGGAGGGCTCAACAACCCTAAGGTCCGTACGTACTGGTCCGAGTCGATTAAAAACAAAAAAACC D S P A S A S R V V G I P G M H D Q A Q L I F V F L

4 Page 4 TAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCA â â â â â â â â 1106 ATCTCTGCCCCAAAGTGGTATAACCGGTCCGACCAGAGGTTGAGGATTAGAGTCCACTAGATGGGTGGAACCGGAGGGT V E T G F H H I G Q A G L Q L L I S G D L P T L A S Q BstXI AATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTAAAATAACTATACCAGCAGGAGGA â â â â â â â â 1185 TTAACGACCCTAATGTCCGCACTTGGTGACGAGGGAAGGGACAGGAAGACTAAAATTTTATTGATATGGTCGTCCTCCT I A G I T G V N H C S L P C P S D F K I T I P A G G AgeI PflMI CGTCCAGACACAGCATAGGCTACCTGGCCATGCCCAACCGGTGGGACATTTGAGTTGCTTGCTTGGCACTGTCCTCTCA â â â â â â â â 1264 GCAGGTCTGTGTCGTATCCGATGGACCGGTACGGGTTGGCCACCCTGTAAACTCAACGAACGAACCGTGACAGGAGAGT R P D T A. A T W P C P T G G T F E L L A W H C P L TGCGTTGGGTCCACTCAGTAGATGCCTGTTGAATTGGGTACGCGGCCAGCTTGGCTGTGGAATGTGTGTCAGTTAGGGT â â â â â â â â 1343 ACGCAACCCAGGTGAGTCATCTACGGACAACTTAACCCATGCGCCGGTCGAACCGACACCTTACACACAGTCAATCCCA M R W V H S V D A C. I G Y A A S L A V E C V S V R V éßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßß SexAI GTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAA â â â â â â â â 1422 CACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTT W K V P R L P S R Q K Y A K H A S Q L V S N Q V W K ßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßß GTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACT â â â â â â â â 1501 CAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGA V P R L P S R Q K Y A K H A S Q L V S N H S P A P N ßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ß

5 Page 5 CCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAG â â â â â â â â 1580 GGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTC S A H P A P N S A Q F R P F S A P W L T N F F Y L C R ßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß SfiI StuI AvrII AGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAA â â â â â â â 1659 TCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGATCCGAAAACGTTTTT G R G R L G L. A I P E V V R R L F W R P R L L Q K ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß XhoI GCTCCTCGAGGAACTGAAAAACCAGAAAGTTAATTCCCTATAGTGAGTCGTATTAAATTCGTAATCATGTCATAGCTGT â â â â â â â â 1738 CGAGGAGCTCCTTGACTTTTTGGTCTTTCAATTAAGGGATATCACTCAGCATAATTTAAGCATTAGTACAGTATCGACA A P R G T E K P E S. F P I V S R I K F V I M S. L TTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGC â â â â â â â â 1817 AAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCCACG F P V. N C Y P L T I P H N I R A G S I K C K A W G A CTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTG â â â â â â â â 1896 GATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGAC.. V S. L T L I A L R S L P A F Q S G N L S C Q L CATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGC â â â â â â â â 1975 GTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCG SapI H.. I G Q R A G R G G L R I G R S S A S S L T D S

6 Page 6 TGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGA â â â â â â â â 2054 ACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCT L R S V V R L R R A V S A H S K A V I R L S T E S G D AflIII PciI TAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTC â â â â â â â â 2133 ATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAG N A G K N M. A K G Q Q K A R N R K K A A L L A F F éßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßß CATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA â â â â â â â â 2212 GTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTT H R L R P P D E H H K N R R S S Q R W R N P T G L. ßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßß GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC â â â â â â â â 2291 CTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCG R Y Q A F P P G S S L V R S P V P T L P L T G Y L S A ßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ß CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCC â â â â â â â â 2370 GAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGG F L P S G S V A L S H S S R C R Y L S S V. V V R S ßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß AAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC â â â â â â â 2449 TTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGG K L G C V H E P P V Q P D R C A L S G N Y R L E S N ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß

7 Page 7 AlwNI CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTAC â â â â â â â â 2528 GCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATG P V R H D L S P L A A A T G N R I S R A R Y V G G A T AGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTT â â â â â â â â 2607 TCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCTTGTCATAAACCATAGACGCGAGACGACTTCGGTCAA E F L K W W P N Y G Y T R R T V F G I C A L L K P V ACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC â â â â â â â â 2686 TGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCG T F G K R V G S S. S G K Q T T A G S G G F F V C K AGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGA â â â â â â â â 2765 TCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCT Q Q I T R R K K G S Q E D P L I F S T G S D A Q W N E AAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGT â â â â â â â â 2844 TTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCA N S R. G I L V M R L S K R I F T. I L L N. K. S TTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAG â â â â â â â â 2923 AAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTC F K S I. S I Y E. T W S D S Y Q C L I S E A P I S éßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßß

8 Page 8 CGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATC â â â â â â â â 3002 GCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAG A I C L F R S S I V A. L P V V. I T T I R E G L P S ßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßß TGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGA â â â â â â â â 3081 ACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCT G P S A A M I P R D P R S P A P D L S A I N Q P A G ßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ß AGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAA â â â â â â â â 3160 TCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATT R A E R R S G P A T L S A S I Q S I N C C R E A R V ßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß GTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTAT â â â â â â â 3239 CATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATA S S S P V N S L R N V V A I A T G I V V S R S S F G M ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß GGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCC â â â â â â â â 3318 CCGAAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTTTTTCGCCAATCGAGG A S F S S G S Q R S R R V T. S P M L C K K A V S S TTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTC â â â â â â â â 3397 AAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAG F G P P I V V R S K L A A V L S L M V M A A L H N S

9 Page 9 ScaI TTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCG â â â â â â â â 3476 AATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGC L T V M P S V R C F S V T G E Y S T K S F. E. C M R GCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATT â â â â â â â â 3555 CGCTGGCTCAACGAGAACGGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAA R P S C S C P A S I R D N T A P H S R T L K V L I I XmnI GGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCAC â â â â â â â â 3634 CCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTACATTGGGTGAGCACGTG G K R S S G R K L S R I L P L L R S S S M. P T R A CCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAA â â â â â â â â 3713 GGTTGACTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTT P N. S S A S F T F T S V S G. A K T G R Q N A A K K éßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßß GGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTAT â â â â â â â â 3792 CCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATA G I R A T R K C. I L I L F L F Q Y Y. S I Y Q G Y ßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßß TGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAG â â â â â â â â 3871 ACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTC C L M S G Y I F E C I. K N K Q I G V P R T F P R K ßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ß

10 F p Page 10 TGCCACCTGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGC â â â â â â â â 3950 ACGGTGGACTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACG V P P D A P C S G A L S A A G V V V T R S V T A T L A ßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß NgoMIV NaeI CAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTA â â â â â â â 4029 GTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGAT S A L A P A P F A F F P S F L A T F A G F P R Q A L ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß AATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTT â â â â â â â â 4108 TTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAA N R G L P L G F R F S A L R H L D P K K L D. G D G DraIII CACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTT â â â â â â â â 4187 GTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAA S R S G P S P.. T V F R P L T L E S T F F N S G L L GTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTAT â â â â â â â â 4266 CAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATA F Q T G T T L N P I S V Y S F D L. G I L P I S A Y TGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTCCATTCGCC â â â â â â â â 4345 ACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCGAATGTTAAAGGTAAGCGG W L K N E L I. Q K F N A N F N K I L T L T I S I R PsiI

11 F p Page 11 ATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGT â â â â â â â â 4424 TAAGTCCGACGCGTTGACAACCCTTCCCGCTAGCCACGCCCGGAGAAGCGATAATGCGGTCGACCGCTTTCCCCCTACA H S G C A T V G K G D R C G P L R Y Y A S W R K G D V GCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTGA â â â â â â â â 4503 CGACGTTCCGCTAATTCAACCCATTGCGGTCCCAAAAGGGTCAGTGCTGCAACATTTTGCTGCCGGTCACGGTTCGACT L Q G D. V G. R Q G F P S H D V V K R R P V P S. éßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßß TCTATACATTGAATCAATATTGGCAATTAGCCATATTAGTCATTGGTTATATAGCATAAATCAATATTGGCTATTGGCC â â â â â â â â 4582 AGATATGTAACTTAGTTATAACCGTTAATCGGTATAATCAGTAACCAATATATCGTATTTAGTTATAACCGATAACCGG S I H. I N I G N. P Y. S L V I. H K S I L A I G ßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßß BsrGI ATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCCAATATGACCGCCATGTTGACATTGA â â â â â â â â 4661 TAACGTATGCAACATAGATATAGTATTATACATGTAAATATAACCGAGTACAGGTTATACTGGCGGTACAACTGTAACT H C I R C I Y I I I C T F I L A H V Q Y D R H V D I D ßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ß SpeI TTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAAC â â â â â â â â 4740 AATAACTGATCAATAATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGCGCAATGTATTG Y. L V I N S N Q L R G H. F I A H I W S S A L H N ßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß TTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGT â â â â â â â 4819 AATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTATCA L R. M A R L A D R P T T P A H. R Q.. R M F P. ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß ßßßßéßßßß

12 F p Page 12 AACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTG â â â â â â â â 4898 TTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCAC. R Q. G L S I D V N G W S I Y G K L P T W Q Y I K C NdeI TATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCT â â â â â â â â 4977 ATAGTATACGGTTCAGGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGA I I C Q V R P L L T S M T V N G P P G I M P S T. P SnaBI TACGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC â â â â â â â â 5056 ATGCCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTG Y G T F L L G S T S T Y. S S L L P W. C G F G S T CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGG â â â â â â â â 5135 GTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACC P M G V D S G L T H G D F Q V S T P L T S M G V C F G CACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCCCGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGT â â â â â â â â 5214 GTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTATTGGGGCGGGGCAACTGCGTTTACCCGCCATCCGCACATGCCA T K I N G T F Q N V V I T P P R. R K W A V G V Y G GG í 5216 CC G éí