3628 &' B: 2018 10((B))32*)10+ NO 4APQRS T5 1,2, 1,2, 1,2,4 1,2 (1 WH #@ ]5,M 610065;2. H 2,M 610065) :; : (CMC)OM (Col)@ ', = W (DCMC), M A B,W M DCMC Col K D DCMC Col #, M #,M!" #$ %& 'N #, DCMC K @15%, ` \ L O MTT b #,DCMC Col ( ) * +I,- &'./,01,DCMC Col 234 K56 &' N Ⅰ% - #^! " <:O636 /0 =>:A 犇犗犐 :10.11896/j.issn.1005 023X.2018.20.024 犘狉犲狆犪狉犪狋犻狅狀狅犳犇犻犪犾犱犲犺狔犱犲犆犪狉犫狅狓狔犿犲狋犺狔犾犆犲犾狌犾狅狊犲 犆狅犾犪犵犲狀犆狅犿狆狅狊犻狋犲犎犲犿狅狊狋犪狋犻犮犕犪狋犲狉犻犪犾 BAIZhongxiang 1,2,DAN Nianhua 1,2,DAN Weihua 1,2,GAO Ran 1,2 (1 KeyLaboratoryofLeatherChemistryandEngineeringofMinistryofEducation,SichuanUniversity,Chengdu610065; 2 BiomedicalEngineeringTechnologyResearchCenter,SichuanUniversity,Chengdu610065) 犃犫狊狋狉犪犮狋 Dialdehydecarboxymethylcelulosesodium (DCMC)wasselectivelyoxidizedutilizingsodiumcarboxymethylcelu lose(cmc)andcolagen (Col)asraw materials,andthecolagenwascross linkedandmodifiedtoobtainanewtypecolagenhemo staticspongedcmc Col.ThemodifiedDCMC Colcompositeswereevaluatedbyinfraredspectroscopy,circulardichroism,diferen tialscanningcalorimetry,atomicforcemicroscopyandscanningelectronmicroscopy.theresultsindicatedthatthethermalstability ofthemodifiedcolagenwassignificantlyimproved,andthetriplehelixstructureofcolagenwasnotdestroyed.thetotalcloting timetestconfirmedthatthehemostaticpropertyofthespongewasoptimalwhenthedosageofdcmcwas15%.theresultsofwater absorption,hemolysisandmtttestresultsdemonstratedthatthepropertiesofdcmc Colcouldmeettherequirementsofhemosta ticmaterialsandithadagoodbiocompatibility.therefore,dcmc Colcompositespongeisanewtypeofhemostaticmaterialswith potentialmedicalapplicationsvalue. 犓犲狔狑狅狉犱狊 carboxymethylcelulose,typeⅠ colagen,modification,hemostaticmaterials 0?@ I! " # P $ 2,% & ' a E C & :T% 5 &.,I] 2 & :( -# ^! % & # ) I 1953 4,L X * % &#- ;20+, 70 4 -,. / ST & ]5P 0]585a% _#^!&L -;804-1 / /% #^!, M 3. $ 2,% ^! & # B S & [1] % #^! 0P _ & >#^! ; ^!G L,&0,E -. #. ^,S L S%& 2 P ^- ;X> 0 % 343 2!, I #56 &7VW,! S 6 Y, -. P 8,S 9 : ; M :, S : > McV& : >,-., + - L # & & [2 4] & % # ^! I A ^ c V - [5 7] F2 7;% ST -, "# & $,, + % N ^!KM_ & J &% - L < "# ' N= H > H? @ F A B B N C ;/ D G % & -, - &% I Y(E, & - < " # C A, -% & [8 11] F, /0 &. I W45, - &F. 3 &. G-P - & XH G8 N _!, : (51473001) :,1994, "#$,#$ %& < E mail:17380140172@163.com =>?:*+,-,, 1956,!",./,!"#$ 01,#$ %& @< ABCDE E mail:dwh5607@263.net
M &' W/ % 3629 2 IH ; N J & C 2 C 3 U ] K-6 + N, W% /& L N.MNO<,;% ST, + L I I &#^! 1 1.1 UV7 N MTT, (,1 SIGMA 4 IV;Ⅰ% (Col),]5 [12] ;RPMI1640 N,.,1 GibcobrlIV;]5, 2 ; 7^!_ (,M 4 PFS 3C ph H,/. I V;Freeze6 P Q,LabconcoIV;MAGNAIR560 c,1 NicoletI V;DSC200 PC, R =S;SPM 9600 $,T G U I V;,TG JascoI V;JSM 5900LV, TG V W 1.2 NO W 4APQRS 56 3.0g & N (CMC), 3 170 ml ph=3&xh] 2,337 Y [\ _ ] ^? 3.975g& IH ]^3110mL ph=3& XH] 2, _`c N ] 2[\,I 37 c< 5h, < 2h,+ (Z I [ 2_ (,P Q + & N (DCMC) Ⅰ% ]^30.1mol/L & H] 2,+\ _5mg/mL&] N ] ^ 30.1 mol/ml H2, \ _ 20 mg/ml D N ] _ ` c % ] 2 [ \, DCMC & _ Col &0% 2% 6% 10% 15% P 20%,3 4 < 24h P Q, + # ] (DCMC Col), ` + 1.3 89XY 1.3.1 & DCMCW DCMC Col W KBr ^, _ c ST`6 c &`6 _400~4000cm -1, a+ _4cm -1,b [c32 1.3.2 D :Q & ^!(DCMC _15%)] 0.1mg/mL, H 1mm c<& 2, ;] ST, _ 190~260nm, - + _ 100nm/min, 3 1.3.3 3~5mgE DCMC & ] F3 2, _20~150, - _10 / min,20ml/min&b$`,8 ; ST` 1.3.4 O a _, $ 64: :Q &] ^! 20μg/mL, 5μL 3 & _/ 5, I 2ST, $ I' ; ST 64: ] M0.3cm 0.3cm &, % 34 /, B $ ' 250 & 1.3.5 7 O\ a + 6 4: + 25 ml G B, @ _ 犿 1; E DCMC & % ], @_ 犿 s;? ] 3 2,24h 8/&, @_ 犿 2; & :, @ _ 犿 3, ]& +( 犘 ) 2 I +: 犘 =( 犿 2- 犿 3- 犿狊 )/( 犿 1- 犿 3) 100% -64: E DCMC &% ] _ 犿 0, ] 3pH _7.4&CH_ (PBS)2,I37 _ 4h :, @ _ 犿 1, ]& +( 犠 )_: 犠 =( 犿 1- 犿 0)/ 犿 0 100% 1.3.6 L ao 8 95 # ] ] + & 6 V: # ] M 1cm 1cm 0.2cm & 4 ", 0.9%& ] 10mL, 337 2 30 min 8 0.2mL& '', 337 2 60min, 5min 2 -; [ 10mL 0.2mL, -; 0.9%& ] 10 ml, 0.2mL,` W/0 / 2, cc I545nm B 6V c ]+( 犡 )& I : 犡 =( 犃 - 犅 )/( 犆 - 犅 ) 100% 2: 犃 ]5 & c ; 犅 -; c ; 犆 - ; c # ] >! & 6 4: E DCMC & #^! 0.02g 34 2,I37 2` 20min, 0.02 ml0.025 mol/l & P 0.2mL&, 37 2 `!, 0 ` 6 3 F T, F 1.3.7 MTT 01: 8 +; &M (L929)_, MTT 7; DCMC Col ^!& G-ST I, - 7 ISO10993 5:2009 2 7B- 2.1 犇犆犕犆 7 PQ 89 _C CMC 6 A & >[\, c ; CMC P DCMC & A S T `6 *1,W CMC,DCMCI1732cm -1 B & > L83 N6 M &, > [13] % & A ;, - _ @! # A B Ⅰ ⅡPⅢ &, > I V /<=
89:; B <=> 3630 2018? 10 @ B A 32 BA 10 C Ⅰ #7 8ÃÄÅÆeÛ 14 15 ü B 2 ³ A 3 5 Ⅰ 5ÑÚ Û Ì 8 s ½ Ï 225nm ø 8! ( N b ~ Z ü 1 1 Ⅱ XLs/1658m 1553m f8nb~? Zü 7?Ô P8 C O Òî ÊÒ N H 8ï SÊ Ò W C N n π 3 5 Ñ Ú Û Ì 8 U B 3 ³ V W C 7 / E N ms 220nm 197nm sü#8( DºNb~ d ä 8Ò î Ê Ò È j, - Ì 8 3 5 Ⅲ X L s / 1240 6& 8ÃÄÅÆeÛ ( rô 8 Z / E 8 ( N 1 m f8nb ~ 7? Ô 7 P 3 5 Ñ 8 C N Ò N H b~ïvk Nb~Ïv q  犚pn d Ø K ] 4ïSÊÒVW 7 CH2 x ù 7 É [ r Ê [ r b~ éz : Ý i < Y ³ æ Z ü Q 7 G 8 ì E v 8ËÒÊÒój,-Ì 3 5 A V W 3 5 B? X [x DCMC G 8 ^ x K F & w x y ` N 7? Ô = Æ L s/3400m 1f 3082m 1f8Nb~ ä?ôù7 8ÐÑgC&^ î 8ÝÞÁÌ 8 8 OH NH2x 8Dº~ ü N H 8 Ò î Ê Ò Ì 8 {27 8Ü Å P Ì 6 & 7 8 5? D º N b ~ YcdOý/ E 8 7 Í v õ 8 Ã Ä Å Æ e Û 7 8Fç ì E i ; g C m n U B 2 P i Î K L Ì ø DCMC 7 835 Ⅰ Ⅱ Ⅲ 8 N b~ï v. K d DCMC C 8wx y = ; ` N w & Ä 8 C N = ;Ñ Y C?Ô7=Æ8Ð Ñ E F & ^ 8 Ý Þ, - Á?Ô7ÑÒ8 DCMC æ Ó C? Ô w [? Ô Æ é = ; Ä[éµ8 C? Ô æ Ó P ý w x y ó s e 2 U Á z & C?Ô8eÛ E Y^ãs DSC TRP_ ( C& (r Ô 8 Z DCMC = ; / E 7 ä 3 5 B 3 DCMC /E7 8 CD TB A 35 B ~ ijî ä Õ =XL& 9 {8 Ö» LÚ YÔÖ~7?Ô³æKF&^ î älêo 16 3 CDp a d 3 v t h45 7 8> E. æ s ^ î G ` a ä Ã Ä Å Æ e Û8 E =Y7 ÿr w# WE Þ ß Ú v d m n 7 8 DSC T B P ^ r s ç? D º ~ B 4 Ù s 35 X L 8 { ~ Z 7 U Ã Ä Å Æ e Û Ç;³ØSeÛ8àY ¾ Ô c d7 8 Ã Ë α á 7 p 3KF^ î 8 â :. Y Z  C 7 8 > E. 3 p3î ~ {! e Û ñ! E è 8 Y 7 8 à ÄÅÆeÛb Æ ÃËÇ ; Ø S 8 α [ á 7 j 37 ã [ H ^ 8 F ç D E _ { À ä õ 17 U B 1 CMC DCMC 8ÜÅ 1 ThT IRp a CMCandDCMC B 4_³VWX î ~ DCMC - > 8 i è z ÿ 7 8 > E. iè  U6 6 G C83 2 d7 Q DC MC =Æ8wxy`N æ Ø å q  7 8 E ± Y ì 7 H^8FçàDE¾ d på üq DCMC?Ô7 G<>8-x 7? Ô 7 G 8 [ x - x â x = Æ wðñ Y_vÏQ7 > E8q B 2 ij DCMC ->8{2+ 8ÜÅ TB 2 ThT IRp a DCMC C mp png w n DCMC n n 2 1245 ¼ëÄ CD æ ÓÄ ûºä @7 ëk eû P 8 ÅÆ e Y õ Ç ; Ø S â Ù b e Û 7 ^ r s α β β ½Ï 197nm øv ^?! 8 N b ~ Z ü π π3 B 4 ij-> DCMC /E7 8 DSC TB 4 DSCu v d png w n d ag
3631 ïðço^½¾è wê+ ø89.< àáâ7 4 e < o 1245 z- Ô6Ø{µY50 3 8 Ô e Û! B 5 Ù Ã7 8 ^ ì e Û ö @ æ ç k m b y 0oÔ 0 V W Î ^ ì X 2 Y : w ^ ì X 2 = ^ÈN ^ìx2]bêo w 8 ^ ì X 2 [ Ê b UÁEF 0. CMEv 508M8 ¾ö@ 7 ^ì=æèée Û! Z = ; 8 7 ^ì e Û Z! ç ê ^ ì 8 v ë E d Ø K ± î ~ DCMC ->8zÿ äçêe 1ìE_zÿ ö ^ x - l x ^ ì _.Ìø&ö^xe Û ] Ì 6 & CMC 7 8 < 5? e Û?Ô>!< DCMC ^ x 7 8 ù 7 [ x ` N w wxyósñ UÁ CMC 8 7eÛíøC 7 ^ìp DCMC 8Ìø ì 7? Ô = Æ ; ³ ö @ 8 1 k Ñ ÐÑ â n Ú v Ä ` N æ 6 8 w x y ó s Ñ µ E DCMC ->b[ Y \ m n B b Ø å ü Q 7 DCMC ç Ö 8?Ô7!Ï oá w î é = ï 8 e Û ± { @ î DC MC 8 ->w(z hi Ã7 8 ^ ì ¾ e Û Ç ; <8 ¾eÛ Y\öw?=ï w 8 Z ¾ e Û v Ï QN kl8«çè8ñð B 6 ij-> DCMC /E7 8 SEM Bñ a 0 b 2 6 d 10 15 20 6 ThSEMmag d png w n d ag a 0 b 2 6 d 10 15 20 5 s @ t45, ïô850+ 7 8? C^r õ C 90 VG!<8? C ³ V 5 0 + q ó Ä < 8 Æ S N 9 80Ð 7 + Ä ä K ö 5 0, - j 3! Â8? C + ÚvÄ 8 çïn, - ³ V Ä ä 9 «UB 7 ³VWX î~ DCMC >8ièz ÿ + 8? Cy. K } e 2 B 6 8 ³ Ó µ B? @ ³ =;<8Ì ø ( 7? Ô 7 = Æ Z 8 e Û ( B 5 ij-> DCMC /E7 8 AM Bñ a 0 b 2 6 d 10 15 20 5 ThAMmag d png w n d ag a 0 b 2 6 Ä ÜÉ 1ì U à 7 + ³ Ó µ B ³ V K L ä ü<> 8 ¾ ò ¾e Û Ûw Áî~ DCMC >8i èzÿ + 8? C Y Z " ± Ä ÿ Ï É m S UB 6 ³VWX î ~ DCMC -> 8z ÿ { 2 5 0 + eføù & DCMC > 20 8 + V Å ä U{2 + 8? Cmæ C 90 VG 250+ 8 õ UB 8 ³VWX î ~ DCMC > 8 z ÿ { 2 + 8 8?` "? _ ( Ä ÿ m ü à 7 + P < > N Ey +G K. K8 } Y³ æz >8 DCMC Y 8 ¾ ò¾eû!m 8? ¾eÛ z & { 2 + 7 Gø x 8 ä ù! Á DCMC q ó & < > 8 ø Y0Ð8N æ 6 j 3 + ³ V Ä ä 9 «N x (+ N E G Á ý [ DCMC 8 Ì ø < > ä ÊO0" 0 " q ó Ê O p T ì 0 " ù&7 qî8 ø x ÿ = + 8? C. K Î Ï ä d 10 15 20
89:; B <=> 3632 N C.K F2? @ : DCMC > 6 3 + 8 N Eh 2018? 10 @ B A 32 BA 10 C 03ÆhÍ 169 50 fh =;<- >ý[ ³ ; üý0ð 7 Ä äcd ±? C 8{ " Î Ï ä N E { op+ 8 5 0 3 Æ ` Á Ñ Ï Ã 7 é Z ü Q DCMC G s<>ø x ] } 0q þä Mäÿ ø C{250 3 P ± Ä " 8? ` _ Ä þ n 0 " «w0! N 0oÔ8 ±é ²3KL 18 YQ_ ë^=;87 î~=;<8zÿ h 7 8ï:Eè( C!<q ]=;<8Ìø{ &7?Ô78ø x ÎÏ50+ 8ø E.K 503ÆdØÑÏ op s5 0ß ]#8 DCMC =;<HQÑ 8_ë^=;< B 7 ij-> DCMC /E7 8? C TB 7 Thp d ymag pngw n d ag B 10 ij-> DCMC /E7 + 8 03Æ 10 Thb d agu a n m d pngw n d ag 7 / t B 8 ij-> DCMC /E7 8N C TB 8 Thwa ab n d p pngw n d ag 6 / @ 2 8Fçé q E Z + - 3 C 8 Ë Ì = ^ ü B 11 ³ {250 3 Y w ^ ì k l 8 z " â # Í v m n :ö^-lx^ì_->ü 0 z ÿ C 20 3 k l 8 é Y z"cms 80 VG 2 MTT kl È E? k ûp1k 0C 38ú Ev 0,- Z 0 Ü k l n Ý 8 õ }Å î~ ö ^ - l x ^ ì _ - > 8 z ÿ k l 8 Þ = X0ÜX28LÚ : 0,- 3 ; Y F ç b éyz"cïvk D Z: ö^ -lx ^ ì _. - > EF^ û2 ^r5 0 3 8 0 C N s 5 V. ü B C 20 3 äéyz"c{ dø üq7 G8 lü[ x 9 ³V W X î ~ DCMC - > 8 z ÿ { 2 + 0 C v ^ G ]hâi ý 4 Y Ü k l Ý Þ î ; 2 + >vú : DCMC - >! 15 3 7 G 8 < > [ x³v ^x! äkf`n ^ xä ù > < ³ Ó 6 > - 38 õ : z ÿ DCMC - >3 7 G[ xy Z `N ý> 8^xÇU [ x ` N Î Ï l ü ^ x > z ÿ Á Ó 6 8 ^xyklfï i Ï D Z : DCMC - >  20 3 7 G8lü [ x u ` N ý > 8 DCMC Î Ï Ó 6 ^ x8>éy [ ;<mn&klz" ÎÏäz"C.K B 9 ij-> DCMC /E7 8 0C 9 Thhm a d y pngw n d ag 7 ^ì³v 0" ~q ó» _ ³ V N 0 " B 11 ij->8 DCMC /E7 + 8 MTT Sef 8 Ê UÁÿM 0ýî U B 10 ³ { 2 5 0 3 11 ThMTT u d 850EædØHQÃ7 ±: DCMC - > 15 3 pngw n d ag
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